Detection of a short CCR5 messenger RNA isoform in human spermatozoa

Radosław Januchowski, Andrzej K. Brȩborowicz, Harold Ofori, Piotr Jedrzejczak, Leszek Pawelczyk, Paweł P. Jagodzinski

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It has recently been reported that the Regulated upon Activation of Normal T-cells Expressed and Secreted (RANTES) chemokine may exhibit a chemotactic effect on sperm. The RANTES chemokine acts on target cells by binding to the CCR5 receptor, which is present on the surface of various cells. Spermatozoa contain a complex repertoire of messenger RNAs (mRNAs) that may provide an insight into past events of spermatogenesis. The type and amount of CCR5 chemokine receptor transcript were investigated in spermatozoa that were isolated by the swim-up method from semen samples of men with normozoospermia. Using reverse transcription and realtime quantitative polymerase chain reaction (RQ-PCR) analysis, we found that the CCR5 mRNA isoform in human spermatozoa consists of exons 3 and 4, and is shorter than the transcript in leukocytes. This CCR5 transcript may represent a more stable mRNA isoform; one that is used to biosynthesize the CCR5 receptor in spermatogenesis or the early stages of embryonic development.

Original languageEnglish (US)
Pages (from-to)757-760
Number of pages4
JournalJournal of Andrology
Volume25
Issue number5
StatePublished - Sep 2004
Externally publishedYes

Fingerprint

RNA Isoforms
CCR5 Receptors
Spermatozoa
Spermatogenesis
Chemokines
T-Lymphocytes
Chemokine Receptors
Semen
Reverse Transcription
Embryonic Development
Exons
Leukocytes
Polymerase Chain Reaction
Messenger RNA

Keywords

  • CCR5
  • mRNA
  • RQ-PCR
  • Sperm

ASJC Scopus subject areas

  • Endocrinology

Cite this

Januchowski, R., Brȩborowicz, A. K., Ofori, H., Jedrzejczak, P., Pawelczyk, L., & Jagodzinski, P. P. (2004). Detection of a short CCR5 messenger RNA isoform in human spermatozoa. Journal of Andrology, 25(5), 757-760.

Detection of a short CCR5 messenger RNA isoform in human spermatozoa. / Januchowski, Radosław; Brȩborowicz, Andrzej K.; Ofori, Harold; Jedrzejczak, Piotr; Pawelczyk, Leszek; Jagodzinski, Paweł P.

In: Journal of Andrology, Vol. 25, No. 5, 09.2004, p. 757-760.

Research output: Contribution to journalArticle

Januchowski, R, Brȩborowicz, AK, Ofori, H, Jedrzejczak, P, Pawelczyk, L & Jagodzinski, PP 2004, 'Detection of a short CCR5 messenger RNA isoform in human spermatozoa', Journal of Andrology, vol. 25, no. 5, pp. 757-760.
Januchowski R, Brȩborowicz AK, Ofori H, Jedrzejczak P, Pawelczyk L, Jagodzinski PP. Detection of a short CCR5 messenger RNA isoform in human spermatozoa. Journal of Andrology. 2004 Sep;25(5):757-760.
Januchowski, Radosław ; Brȩborowicz, Andrzej K. ; Ofori, Harold ; Jedrzejczak, Piotr ; Pawelczyk, Leszek ; Jagodzinski, Paweł P. / Detection of a short CCR5 messenger RNA isoform in human spermatozoa. In: Journal of Andrology. 2004 ; Vol. 25, No. 5. pp. 757-760.
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