Detection of a mediator derived from endotoxin-stimulated macrophages that induces the acute phase serum amyloid A response in mice

J. D. Sipe, S. N. Vogel, J. L. Ryan, K. P. McAdam, David L. Rosenstreich

Research output: Contribution to journalArticle

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Abstract

The mechanism by which LPS stimulates an acute phase serum amyloid A (SAA) response in C3H mice has been studied. A factor (SAA inducer) appears in the blood of C3H/HeN (lipopolysaccharide [LPS]-sensitive) mice ~ 1 hr after administration of LPS, which, when passively administered, can induce C3H/HeJ mice to produce SAA although they are resistant to the LPS itself. SAA inducer has been detected in the culture medium of LPS treated C3H/HeN macrophages but not spleen cells. Thus, 2 stages in the induction of the acute phase SAA response are now recognized: A latent period of 2-3 hr during which the SAA concentration remains at baseline values and in which SAA inducer appears, and the period of synthesis of SAA which lasts for ~24 hr past induction. It is proposed that a macrophage response to LPS is responsible for production of the serum mediator which induces SAA synthesis.

Original languageEnglish (US)
Pages (from-to)597-606
Number of pages10
JournalJournal of Experimental Medicine
Volume150
Issue number3
DOIs
StatePublished - 1979
Externally publishedYes

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Serum Amyloid A Protein
Endotoxins
Macrophages
Lipopolysaccharides
Inbred C3H Mouse
Hairless Mouse
Culture Media
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Detection of a mediator derived from endotoxin-stimulated macrophages that induces the acute phase serum amyloid A response in mice. / Sipe, J. D.; Vogel, S. N.; Ryan, J. L.; McAdam, K. P.; Rosenstreich, David L.

In: Journal of Experimental Medicine, Vol. 150, No. 3, 1979, p. 597-606.

Research output: Contribution to journalArticle

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