Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles

Sara H. Rouhanifard; Ran Xie; Guoxin Zhang; Xiaoming Sun; Xing Chen; Peng Wu

doi:10.1021/bm3007506

Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles

Sara H. Rouhanifard, Ran Xie, Guoxin Zhang, Xiaoming Sun, Xing Chen, Peng Wu

Biochemistry

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (Le^X). Here, we report the fabrication of magnetic nanoparticles coated with multivalent Le^X glycans using Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.

Original language	English (US)
Pages (from-to)	3039-3045
Number of pages	7
Journal	Biomacromolecules
Volume	13
Issue number	10
DOIs	https://doi.org/10.1021/bm3007506
State	Published - Oct 8 2012

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1021/bm3007506

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AU - Rouhanifard, Sara H.

AU - Xie, Ran

AU - Zhang, Guoxin

AU - Sun, Xiaoming

AU - Chen, Xing

AU - Wu, Peng

PY - 2012/10/8

Y1 - 2012/10/8

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AB - dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (LeX). Here, we report the fabrication of magnetic nanoparticles coated with multivalent LeX glycans using Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.

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Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles

Abstract

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