TY - JOUR
T1 - Detection and enumeration of colonic mucosal cells in amniotic fluid using a colon epithelial‐specific monoclonal antibody
AU - Chitayat, David
AU - Marion, Robert W.
AU - Squillante, Linda
AU - Kalousek, Dagmar K.
AU - Das, Kiron M.
PY - 1990/11
Y1 - 1990/11
N2 - Since its introduction, prenatal diagnosis of chromosomal and metabolic disorder by mid‐trimester amniocentesis has relied upon the use of a mixture of fetal cells obtained from amniotic fluid. Little knowledge has been gained in the sorting of these cells for diagnosis of tissue‐specific disorders. In an attempt to determine the contribution of fetal colonic mucosal cells to the overall amniocyte population, we used the colonic epithelial‐specific monoclonal antibody (MC‐Ab) 7E12H12, IgM isotype. Specimens of the small intestine, colon, buccal mucosa, kidney, urinary bladder, and umbilical cord were obtained from electively aborted normal fetuses of 12–28 weeks' gestation. All of these specimens were examined with 7E12H12 by the immunoperoxidase technique. The MC‐Ab reacted with the colonic epithelial cells but not with any of the other tissues. In addition, 40 amniotic fluid samples obtained from women between 16 and 18 weeks of gestation, who underwent amniocentesis because of advanced maternal age, were tested using a fluorescent activated cell sorter. Among the amniotic fluid specimens examined, 18·4 ± 10·3 percent cells reacted with 7E12H12. Double immunofluorescence studies revealed that all Mc‐Ab‐stained cells contained secretory component, confirming that they were epithelial in origin. All fetuses whose amniotic fluid was analysed had normal karyotypes and amniotic fluid alpha‐fetoprctein levels that were also normal. This study demonstrates that cell‐specific Mc‐Ab can be used to detect colon cells in the amniotic fluid and that colon cells contribute significant numbers in the mixture of amniotic fluid cells. This technique could be helpful in the prenatal diagnosis of disorders in which the flow of amniotic fluid through the fetal intestine is impaired, such as cystic fibrosis, imperforate anus, Hirschsprung aganglionic megacolon, and intestinal atresia.
AB - Since its introduction, prenatal diagnosis of chromosomal and metabolic disorder by mid‐trimester amniocentesis has relied upon the use of a mixture of fetal cells obtained from amniotic fluid. Little knowledge has been gained in the sorting of these cells for diagnosis of tissue‐specific disorders. In an attempt to determine the contribution of fetal colonic mucosal cells to the overall amniocyte population, we used the colonic epithelial‐specific monoclonal antibody (MC‐Ab) 7E12H12, IgM isotype. Specimens of the small intestine, colon, buccal mucosa, kidney, urinary bladder, and umbilical cord were obtained from electively aborted normal fetuses of 12–28 weeks' gestation. All of these specimens were examined with 7E12H12 by the immunoperoxidase technique. The MC‐Ab reacted with the colonic epithelial cells but not with any of the other tissues. In addition, 40 amniotic fluid samples obtained from women between 16 and 18 weeks of gestation, who underwent amniocentesis because of advanced maternal age, were tested using a fluorescent activated cell sorter. Among the amniotic fluid specimens examined, 18·4 ± 10·3 percent cells reacted with 7E12H12. Double immunofluorescence studies revealed that all Mc‐Ab‐stained cells contained secretory component, confirming that they were epithelial in origin. All fetuses whose amniotic fluid was analysed had normal karyotypes and amniotic fluid alpha‐fetoprctein levels that were also normal. This study demonstrates that cell‐specific Mc‐Ab can be used to detect colon cells in the amniotic fluid and that colon cells contribute significant numbers in the mixture of amniotic fluid cells. This technique could be helpful in the prenatal diagnosis of disorders in which the flow of amniotic fluid through the fetal intestine is impaired, such as cystic fibrosis, imperforate anus, Hirschsprung aganglionic megacolon, and intestinal atresia.
KW - Colonic cells
KW - Intestinal malformations
KW - Monoclonal antibodies
KW - Prenatal diagnosis
UR - http://www.scopus.com/inward/record.url?scp=0025669962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025669962&partnerID=8YFLogxK
U2 - 10.1002/pd.1970101106
DO - 10.1002/pd.1970101106
M3 - Article
C2 - 2284274
AN - SCOPUS:0025669962
SN - 0197-3851
VL - 10
SP - 725
EP - 732
JO - Prenatal Diagnosis
JF - Prenatal Diagnosis
IS - 11
ER -