Design and use of fluorescent fusion proteins in cell biology.

Erik Snapp

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.

Original languageEnglish (US)
Pages (from-to)Unit 21.4
JournalCurrent protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]
VolumeChapter 21
StatePublished - Jul 2005

ASJC Scopus subject areas

  • Cell Biology

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