This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.
|Original language||English (US)|
|Pages (from-to)||Unit 21.4|
|Journal||Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]|
|State||Published - Jul 2005|
ASJC Scopus subject areas
- Cell Biology