Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

Nebojsa S. Marinkovic, Aleksandar R. Adzic, Michael Sullivan, Kevin Kovacs, Lisa M. Miller, Denis L. Rousseau, Syun-Ru Yeh, Mark R. Chance

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

Original languageEnglish (US)
Pages (from-to)4057-4060
Number of pages4
JournalReview of Scientific Instruments
Volume71
Issue number11
StatePublished - Nov 2000

Fingerprint

Infrared radiation
Kinetics
Infrared lasers
cytochromes
kinetics
cells
infrared lasers
folding
chemical reactions
Fourier transforms
Diodes
examination
diodes
Proteins
Wavelength
wavelengths

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Instrumentation

Cite this

Marinkovic, N. S., Adzic, A. R., Sullivan, M., Kovacs, K., Miller, L. M., Rousseau, D. L., ... Chance, M. R. (2000). Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy. Review of Scientific Instruments, 71(11), 4057-4060.

Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy. / Marinkovic, Nebojsa S.; Adzic, Aleksandar R.; Sullivan, Michael; Kovacs, Kevin; Miller, Lisa M.; Rousseau, Denis L.; Yeh, Syun-Ru; Chance, Mark R.

In: Review of Scientific Instruments, Vol. 71, No. 11, 11.2000, p. 4057-4060.

Research output: Contribution to journalArticle

Marinkovic, NS, Adzic, AR, Sullivan, M, Kovacs, K, Miller, LM, Rousseau, DL, Yeh, S-R & Chance, MR 2000, 'Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy', Review of Scientific Instruments, vol. 71, no. 11, pp. 4057-4060.
Marinkovic, Nebojsa S. ; Adzic, Aleksandar R. ; Sullivan, Michael ; Kovacs, Kevin ; Miller, Lisa M. ; Rousseau, Denis L. ; Yeh, Syun-Ru ; Chance, Mark R. / Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy. In: Review of Scientific Instruments. 2000 ; Vol. 71, No. 11. pp. 4057-4060.
@article{5a6d61383e384fe59f5e10137f731a67,
title = "Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy",
abstract = "A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.",
author = "Marinkovic, {Nebojsa S.} and Adzic, {Aleksandar R.} and Michael Sullivan and Kevin Kovacs and Miller, {Lisa M.} and Rousseau, {Denis L.} and Syun-Ru Yeh and Chance, {Mark R.}",
year = "2000",
month = "11",
language = "English (US)",
volume = "71",
pages = "4057--4060",
journal = "Review of Scientific Instruments",
issn = "0034-6748",
publisher = "American Institute of Physics Publising LLC",
number = "11",

}

TY - JOUR

T1 - Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

AU - Marinkovic, Nebojsa S.

AU - Adzic, Aleksandar R.

AU - Sullivan, Michael

AU - Kovacs, Kevin

AU - Miller, Lisa M.

AU - Rousseau, Denis L.

AU - Yeh, Syun-Ru

AU - Chance, Mark R.

PY - 2000/11

Y1 - 2000/11

N2 - A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

AB - A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

UR - http://www.scopus.com/inward/record.url?scp=0010602618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0010602618&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0010602618

VL - 71

SP - 4057

EP - 4060

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

SN - 0034-6748

IS - 11

ER -