Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

Nebojsa S. Marinkovic; Aleksandar R. Adzic; Michael Sullivan; Kevin Kovacs; Lisa M. Miller; Denis L. Rousseau; Syun Ru Yeh; Mark R. Chance

doi:10.1063/1.1319342

Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

Nebojsa S. Marinkovic, Aleksandar R. Adzic, Michael Sullivan, Kevin Kovacs, Lisa M. Miller, Denis L. Rousseau, Syun Ru Yeh, Mark R. Chance

Biochemistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

Original language	English (US)
Pages (from-to)	4057-4060
Number of pages	4
Journal	Review of Scientific Instruments
Volume	71
Issue number	11
DOIs	https://doi.org/10.1063/1.1319342
State	Published - Nov 2000

ASJC Scopus subject areas

Instrumentation

Access to Document

10.1063/1.1319342

Cite this

@article{5a6d61383e384fe59f5e10137f731a67,

title = "Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy",

abstract = "A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.",

author = "Marinkovic, {Nebojsa S.} and Adzic, {Aleksandar R.} and Michael Sullivan and Kevin Kovacs and Miller, {Lisa M.} and Rousseau, {Denis L.} and Yeh, {Syun Ru} and Chance, {Mark R.}",

year = "2000",

month = nov,

doi = "10.1063/1.1319342",

language = "English (US)",

volume = "71",

pages = "4057--4060",

journal = "Review of Scientific Instruments",

issn = "0034-6748",

publisher = "American Institute of Physics Publising LLC",

number = "11",

}

TY - JOUR

T1 - Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

AU - Marinkovic, Nebojsa S.

AU - Adzic, Aleksandar R.

AU - Sullivan, Michael

AU - Kovacs, Kevin

AU - Miller, Lisa M.

AU - Rousseau, Denis L.

AU - Yeh, Syun Ru

AU - Chance, Mark R.

PY - 2000/11

Y1 - 2000/11

N2 - A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

AB - A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

UR - http://www.scopus.com/inward/record.url?scp=0010602618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0010602618&partnerID=8YFLogxK

U2 - 10.1063/1.1319342

DO - 10.1063/1.1319342

M3 - Article

AN - SCOPUS:0010602618

SN - 0034-6748

VL - 71

SP - 4057

EP - 4060

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

IS - 11

ER -

Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this