Design and implementation of a rapid-mixer flow cell for time-resolved infrared microspectroscopy

Nebojsa S. Marinkovic, Aleksandar R. Adzic, Michael Sullivan, Kevin Kovacs, Lisa M. Miller, Denis L. Rousseau, Syun Ru Yeh, Mark R. Chance

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A rapid mixer for the analysis of reactions in the millisecond and submillisecond time domains by Fourier-transform infrared microspectroscopy has been constructed. The cell was tested by examination of cytochrome-c folding kinetics. The device allows collection of full infrared spectral data on millisecond and faster time scales subsequent to chemical jump reaction initiation. The data quality is sufficiently good such that spectral fitting techniques could be applied to analysis of the data. Thus, this method provides an advantage over kinetic measurements at single wavelengths using infrared laser or diode sources, particularly where band overlap exists.

Original languageEnglish (US)
Pages (from-to)4057-4060
Number of pages4
JournalReview of Scientific Instruments
Volume71
Issue number11
DOIs
StatePublished - Nov 2000

ASJC Scopus subject areas

  • Instrumentation

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