TY - JOUR
T1 - Design and application of 2-D DGGE-based gene mutational scanning tests
AU - Van Orsouw, Nathalie J.
AU - Vijg, Jan
N1 - Funding Information:
We thank Dr C. Eng for critically reading the manuscript, and we would like to acknowledge Drs C. Eng and I. Sigalas and R.K. Dhanda, R.-D. Rines and W. Smith for contributing to the work described in this paper. We are also grateful to Dr L. Lerman for his helpful suggestions during the development of TDGS. This research was supported by the Massachusetts Department of Public Health (3408799DO47), the Harvard Nathan Shock Center of Excellence in the Basic Biology of Aging (1P30AG13314-01) and research funds from Beltronics Inc., Newton, MA.
PY - 1999/2
Y1 - 1999/2
N2 - Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.
AB - Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.
KW - 2-D DGGE
KW - Gene mutational scanning tests
KW - PCR
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U2 - 10.1016/S1050-3862(98)00028-X
DO - 10.1016/S1050-3862(98)00028-X
M3 - Article
C2 - 10084116
AN - SCOPUS:0032971221
SN - 1050-3862
VL - 14
SP - 205
EP - 213
JO - Genetic Analysis - Biomolecular Engineering
JF - Genetic Analysis - Biomolecular Engineering
IS - 5-6
ER -
