Design and application of 2-D DGGE-based gene mutational scanning tests

Nathalie J. Van Orsouw, Jan Vijg

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalGenetic Analysis - Biomolecular Engineering
Volume14
Issue number5-6
DOIs
StatePublished - Feb 1 1999
Externally publishedYes

Keywords

  • 2-D DGGE
  • Gene mutational scanning tests
  • PCR

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

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