Description of a novel panallergen of cross-reactivity between moulds and foods

Inmaculada Herrera-Mozo, Beatriz Ferrer, Jose Luís Rodriguez-Sanchez, Cándido Juarez

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The present investigation is undertaken to demonstrate a novel cross-reactivity between aeroallergens (moulds fungi imperfecti) and allergens from foods (spinach and mushroom Agaricus bisporus). We have performed a dual study in vivo and in vitro, in a population of atopic patients. Data from in vivo tests performed with spinach and mushroom have been statistically analysed. To the in vitro assays, mushroom and spinach extracts have been obtained, and sera from moulds allergic patients analysed by means of IgE-immunoblott assays. Inhibition experiments have been also performed to study a possible relation between proteins. Statistical analysis of data showed a relation between allergenicity to moulds (Alternaria alternata, Cladosporium herbarum and/or Aspergillus fumigatus), and positive skin prick tests with mushroom and/or spinach. The immunoblotts performed showed that seven moulds allergic patients had a strong recognition of a protein with a molecular weight of about 30 kD present both in spinach and mushroom extracts, and by means of inhibition assays we could determine that these two proteins were related. This study demonstrates the existence of a new allergen responsible for cross reactivity between moulds and two frequently consumed foods, mushroom and spinach. We conclude that a novel cross-reactive allergen between aeroallergens and foods has been identified.

Original languageEnglish (US)
Pages (from-to)181-197
Number of pages17
JournalImmunological Investigations
Volume35
Issue number2
DOIs
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

Spinacia oleracea
Agaricales
Fungi
Food
Allergens
Mitosporic Fungi
Agaricus
Cladosporium
Alternaria
Statistical Data Interpretation
Proteins
Aspergillus fumigatus
Skin Tests
Immunoglobulin E
Molecular Weight
Serum
Population

Keywords

  • Cross-reactivity
  • Food allergy
  • Moulds allergy
  • Mushroom
  • Panallergen
  • Spinach

ASJC Scopus subject areas

  • Immunology

Cite this

Description of a novel panallergen of cross-reactivity between moulds and foods. / Herrera-Mozo, Inmaculada; Ferrer, Beatriz; Rodriguez-Sanchez, Jose Luís; Juarez, Cándido.

In: Immunological Investigations, Vol. 35, No. 2, 01.05.2006, p. 181-197.

Research output: Contribution to journalArticle

Herrera-Mozo, Inmaculada ; Ferrer, Beatriz ; Rodriguez-Sanchez, Jose Luís ; Juarez, Cándido. / Description of a novel panallergen of cross-reactivity between moulds and foods. In: Immunological Investigations. 2006 ; Vol. 35, No. 2. pp. 181-197.
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