Deregulation of DUX4 and ERG in acute lymphoblastic leukemia

for the St. Jude Children's Research Hospital?Washington University Pediatric Cancer Genome Project

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Chromosomal rearrangements deregulating hematopoietic transcription factors are common in acute lymphoblastic leukemia (ALL). Here we show that deregulation of the homeobox transcription factor gene DUX4 and the ETS transcription factor gene ERG is a hallmark of a subtype of B-progenitor ALL that comprises up to 7% of B-ALL. DUX4 rearrangement and overexpression was present in all cases and was accompanied by transcriptional deregulation of ERG, expression of a novel ERG isoform, ERGalt, and frequent ERG deletion. ERGalt uses a non-canonical first exon whose transcription was initiated by DUX4 binding. ERGalt retains the DNA-binding and transactivation domains of ERG, but it inhibits wild-type ERG transcriptional activity and is transforming. These results illustrate a unique paradigm of transcription factor deregulation in leukemia in which DUX4 deregulation results in loss of function of ERG, either by deletion or induced expression of an isoform that is a dominant-negative inhibitor of wild-type ERG function.

Original languageEnglish (US)
Pages (from-to)1481-1489
Number of pages9
JournalNature Genetics
Volume48
Issue number12
DOIs
StatePublished - Dec 1 2016

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
Transcription Factors
Protein Isoforms
Homeobox Genes
Transcriptional Activation
Genes
Exons
Leukemia
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

for the St. Jude Children's Research Hospital?Washington University Pediatric Cancer Genome Project (2016). Deregulation of DUX4 and ERG in acute lymphoblastic leukemia. Nature Genetics, 48(12), 1481-1489. https://doi.org/10.1038/ng.3691

Deregulation of DUX4 and ERG in acute lymphoblastic leukemia. / for the St. Jude Children's Research Hospital?Washington University Pediatric Cancer Genome Project.

In: Nature Genetics, Vol. 48, No. 12, 01.12.2016, p. 1481-1489.

Research output: Contribution to journalArticle

for the St. Jude Children's Research Hospital?Washington University Pediatric Cancer Genome Project 2016, 'Deregulation of DUX4 and ERG in acute lymphoblastic leukemia', Nature Genetics, vol. 48, no. 12, pp. 1481-1489. https://doi.org/10.1038/ng.3691
for the St. Jude Children's Research Hospital?Washington University Pediatric Cancer Genome Project. Deregulation of DUX4 and ERG in acute lymphoblastic leukemia. Nature Genetics. 2016 Dec 1;48(12):1481-1489. https://doi.org/10.1038/ng.3691
for the St. Jude Children's Research Hospital?Washington University Pediatric Cancer Genome Project. / Deregulation of DUX4 and ERG in acute lymphoblastic leukemia. In: Nature Genetics. 2016 ; Vol. 48, No. 12. pp. 1481-1489.
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