TY - JOUR
T1 - Depressed myosin ATPase activity in hearts of myopathic hamsters
T2 - Dissociation from neutral protease activity
AU - Bhan, Ashok
AU - Malhotra, Ashwani
AU - Hatcher, Victor B.
AU - Sonnenblick, Edmund H.
AU - Scheuer, James
PY - 1978/1/1
Y1 - 1978/1/1
N2 - Myosin was isolated from the hearts of 210-day-old genetically myopathic Syrian hamsters (Bio 53:58) and its properties compared to the myosin from random bred controls of the same age. The Ca2+ ATPase and the total-SH content did not differ significantly in the two groups. Myosin from myopathic animals showed a loss of LC2. Analyses of myofibrils and actomyosin revealed that the LC2 was present in these preparations from the myopathic animals and the loss of LC2 was taking place during the purification of myosin. Despite the presence of LC2 in the myofibrils and actomyosin, the corresponding ATPase activities were depressed in the myopathic hearts. The loss of LC2 in myopathic cardiac myosin was found to be due to high levels of neutral protease activity co-purifying with myosin. The protease fraction from myopathic hearts degraded the cardiac LC2 of control hamster and dog cardiac myosin, however, the Ca++ ATPase remained unchanged.
AB - Myosin was isolated from the hearts of 210-day-old genetically myopathic Syrian hamsters (Bio 53:58) and its properties compared to the myosin from random bred controls of the same age. The Ca2+ ATPase and the total-SH content did not differ significantly in the two groups. Myosin from myopathic animals showed a loss of LC2. Analyses of myofibrils and actomyosin revealed that the LC2 was present in these preparations from the myopathic animals and the loss of LC2 was taking place during the purification of myosin. Despite the presence of LC2 in the myofibrils and actomyosin, the corresponding ATPase activities were depressed in the myopathic hearts. The loss of LC2 in myopathic cardiac myosin was found to be due to high levels of neutral protease activity co-purifying with myosin. The protease fraction from myopathic hearts degraded the cardiac LC2 of control hamster and dog cardiac myosin, however, the Ca++ ATPase remained unchanged.
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U2 - 10.1016/0040-4020(78)80095-7
DO - 10.1016/0040-4020(78)80095-7
M3 - Article
C2 - 151748
VL - 10
SP - 769
EP - 772
JO - Journal of Trace Elements in Medicine and Biology
JF - Journal of Trace Elements in Medicine and Biology
SN - 0946-672X
IS - 8
ER -