Depleted uranium is not toxic to rat brain endothelial (RBE4) cells

Allison W. Dobson; Anna K. Lack; Keith M. Erikson; Michael Aschner

doi:10.1385/BTER:110:1:61

Depleted uranium is not toxic to rat brain endothelial (RBE4) cells

Allison W. Dobson, Anna K. Lack, Keith M. Erikson, Michael Aschner

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Studies on Gulf War veterans with depleted uranium (DU) fragments embedded in their soft tissues have led to suggestions of possible DU-induced neurotoxicity. We investigated DU uptake into cultured rat brain endothelial cells (RBE4). Following the determination that DU readily enters RBE4 cells, cytotoxic effects were analyzed using assays for cell volume increase, heat shock protein 90 (Hsp90) expression, 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactate dehydrogenase (LDH) activity. The results of these studies show that uptake of the U ₃O₈ uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these cells as detected by common biomarkers. Thus, the present experimental paradigm is rather reassuring and provides no indication for overt cytotoxicity in endothelial cells exposed to DU.

Original language	English (US)
Pages (from-to)	61-72
Number of pages	12
Journal	Biological Trace Element Research
Volume	110
Issue number	1
DOIs	https://doi.org/10.1385/BTER:110:1:61
State	Published - 2006
Externally published	Yes

Keywords

Blood-brain barrier
Depleted uranium (DU)
Endothelium
Heavey metal toxicity

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Clinical Biochemistry
Inorganic Chemistry
Biochemistry, medical

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1385/BTER:110:1:61

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@article{d4039c34184f47f884c14ee4331d1120,

title = "Depleted uranium is not toxic to rat brain endothelial (RBE4) cells",

abstract = "Studies on Gulf War veterans with depleted uranium (DU) fragments embedded in their soft tissues have led to suggestions of possible DU-induced neurotoxicity. We investigated DU uptake into cultured rat brain endothelial cells (RBE4). Following the determination that DU readily enters RBE4 cells, cytotoxic effects were analyzed using assays for cell volume increase, heat shock protein 90 (Hsp90) expression, 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactate dehydrogenase (LDH) activity. The results of these studies show that uptake of the U 3O8 uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these cells as detected by common biomarkers. Thus, the present experimental paradigm is rather reassuring and provides no indication for overt cytotoxicity in endothelial cells exposed to DU.",

keywords = "Blood-brain barrier, Depleted uranium (DU), Endothelium, Heavey metal toxicity",

author = "Dobson, {Allison W.} and Lack, {Anna K.} and Erikson, {Keith M.} and Michael Aschner",

note = "Funding Information: This work was supported by Grant DAMD17-01-1-0685 (to MA) from the US Department of Defense (DoD).",

year = "2006",

doi = "10.1385/BTER:110:1:61",

language = "English (US)",

volume = "110",

pages = "61--72",

journal = "Biological Trace Element Research",

issn = "0163-4984",

publisher = "Humana Press",

number = "1",

}

TY - JOUR

T1 - Depleted uranium is not toxic to rat brain endothelial (RBE4) cells

AU - Dobson, Allison W.

AU - Lack, Anna K.

AU - Erikson, Keith M.

AU - Aschner, Michael

N1 - Funding Information: This work was supported by Grant DAMD17-01-1-0685 (to MA) from the US Department of Defense (DoD).

PY - 2006

Y1 - 2006

N2 - Studies on Gulf War veterans with depleted uranium (DU) fragments embedded in their soft tissues have led to suggestions of possible DU-induced neurotoxicity. We investigated DU uptake into cultured rat brain endothelial cells (RBE4). Following the determination that DU readily enters RBE4 cells, cytotoxic effects were analyzed using assays for cell volume increase, heat shock protein 90 (Hsp90) expression, 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactate dehydrogenase (LDH) activity. The results of these studies show that uptake of the U 3O8 uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these cells as detected by common biomarkers. Thus, the present experimental paradigm is rather reassuring and provides no indication for overt cytotoxicity in endothelial cells exposed to DU.

AB - Studies on Gulf War veterans with depleted uranium (DU) fragments embedded in their soft tissues have led to suggestions of possible DU-induced neurotoxicity. We investigated DU uptake into cultured rat brain endothelial cells (RBE4). Following the determination that DU readily enters RBE4 cells, cytotoxic effects were analyzed using assays for cell volume increase, heat shock protein 90 (Hsp90) expression, 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactate dehydrogenase (LDH) activity. The results of these studies show that uptake of the U 3O8 uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these cells as detected by common biomarkers. Thus, the present experimental paradigm is rather reassuring and provides no indication for overt cytotoxicity in endothelial cells exposed to DU.

KW - Blood-brain barrier

KW - Depleted uranium (DU)

KW - Endothelium

KW - Heavey metal toxicity

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SN - 0163-4984

VL - 110

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JO - Biological Trace Element Research

JF - Biological Trace Element Research

IS - 1

ER -

Depleted uranium is not toxic to rat brain endothelial (RBE4) cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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