Depleted uranium is not toxic to rat brain endothelial (RBE4) cells

Allison W. Dobson, Anna K. Lack, Keith M. Erikson, Michael Aschner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Studies on Gulf War veterans with depleted uranium (DU) fragments embedded in their soft tissues have led to suggestions of possible DU-induced neurotoxicity. We investigated DU uptake into cultured rat brain endothelial cells (RBE4). Following the determination that DU readily enters RBE4 cells, cytotoxic effects were analyzed using assays for cell volume increase, heat shock protein 90 (Hsp90) expression, 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction, and lactate dehydrogenase (LDH) activity. The results of these studies show that uptake of the U 3O8 uranyl chloride form of DU into RBE4 cells is efficient, but there are little or no resulting cytotoxic effects on these cells as detected by common biomarkers. Thus, the present experimental paradigm is rather reassuring and provides no indication for overt cytotoxicity in endothelial cells exposed to DU.

Original languageEnglish (US)
Pages (from-to)61-72
Number of pages12
JournalBiological Trace Element Research
Volume110
Issue number1
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Uranium
Poisons
Endothelial cells
Rats
Brain
Endothelial Cells
Gulf War
HSP90 Heat-Shock Proteins
Biomarkers
Veterans
Cytotoxicity
Cell Size
L-Lactate Dehydrogenase
Assays
Tissue

Keywords

  • Blood-brain barrier
  • Depleted uranium (DU)
  • Endothelium
  • Heavey metal toxicity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Depleted uranium is not toxic to rat brain endothelial (RBE4) cells. / Dobson, Allison W.; Lack, Anna K.; Erikson, Keith M.; Aschner, Michael.

In: Biological Trace Element Research, Vol. 110, No. 1, 2006, p. 61-72.

Research output: Contribution to journalArticle

Dobson, Allison W. ; Lack, Anna K. ; Erikson, Keith M. ; Aschner, Michael. / Depleted uranium is not toxic to rat brain endothelial (RBE4) cells. In: Biological Trace Element Research. 2006 ; Vol. 110, No. 1. pp. 61-72.
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