Dendritic cell-mediated in vivo bone resorption

Radhashree Maitra, Antonia Follenzi, Arash Yaghoobian, Cristina Montagna, Simone Merlin, Elvira S. Cannizzo, John A. Hardin, Neil Cobelli, E. Richard Stanley, Laura Santambrogio

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Osteoclasts are resident cells of the bone that are primarily involved in the physiological and pathological remodeling of this tissue. Mature osteoclasts are multinucleated giant cells that are generated from the fusion of circulating precursors originating from the monocyte/macrophage lineage. During inflammatory bone conditions in vivo, de novo osteoclastogenesis is observed but it is currently unknown whether, besides increased osteoclast differentiation from undifferentiated precursors, other cell types can generate a multinucleated giant cell phenotype with bone resorbing activity. In this study, an animal model of calvaria-induced aseptic osteolysis was used to analyze possible bone resorption capabilities of dendritic cells (DCs).We determined by FACS analysis and confocal microscopy that injected GFP-labeled immature DCs were readily recruited to the site of osteolysis. Upon recruitment, the cathepsin K-positive DCs were observed in bone-resorbing pits. Additionally, chromosomal painting identified nuclei from female DCs, previously injected into a male recipient, among the nuclei of giant cells at sites of osteolysis. Finally, osteolysis was also observed upon recruitment of CD11c-GFP conventional DCs in Csf1r -/- mice, which exhibit a severe depletion of resident osteoclasts and tissue macrophages. Altogether, our analysis indicates that DCs may have an important role in bone resorption associated with various inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)1485-1491
Number of pages7
JournalJournal of Immunology
Volume185
Issue number3
DOIs
StatePublished - Aug 1 2010

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Bone Resorption
Dendritic Cells
Osteolysis
Osteoclasts
Giant Cells
Bone and Bones
Macrophages
Cathepsin K
Paintings
Cell Fusion
Osteogenesis
Skull
Confocal Microscopy
Monocytes
Animal Models
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Maitra, R., Follenzi, A., Yaghoobian, A., Montagna, C., Merlin, S., Cannizzo, E. S., ... Santambrogio, L. (2010). Dendritic cell-mediated in vivo bone resorption. Journal of Immunology, 185(3), 1485-1491. https://doi.org/10.4049/jimmunol.0903560

Dendritic cell-mediated in vivo bone resorption. / Maitra, Radhashree; Follenzi, Antonia; Yaghoobian, Arash; Montagna, Cristina; Merlin, Simone; Cannizzo, Elvira S.; Hardin, John A.; Cobelli, Neil; Stanley, E. Richard; Santambrogio, Laura.

In: Journal of Immunology, Vol. 185, No. 3, 01.08.2010, p. 1485-1491.

Research output: Contribution to journalArticle

Maitra, R, Follenzi, A, Yaghoobian, A, Montagna, C, Merlin, S, Cannizzo, ES, Hardin, JA, Cobelli, N, Stanley, ER & Santambrogio, L 2010, 'Dendritic cell-mediated in vivo bone resorption', Journal of Immunology, vol. 185, no. 3, pp. 1485-1491. https://doi.org/10.4049/jimmunol.0903560
Maitra R, Follenzi A, Yaghoobian A, Montagna C, Merlin S, Cannizzo ES et al. Dendritic cell-mediated in vivo bone resorption. Journal of Immunology. 2010 Aug 1;185(3):1485-1491. https://doi.org/10.4049/jimmunol.0903560
Maitra, Radhashree ; Follenzi, Antonia ; Yaghoobian, Arash ; Montagna, Cristina ; Merlin, Simone ; Cannizzo, Elvira S. ; Hardin, John A. ; Cobelli, Neil ; Stanley, E. Richard ; Santambrogio, Laura. / Dendritic cell-mediated in vivo bone resorption. In: Journal of Immunology. 2010 ; Vol. 185, No. 3. pp. 1485-1491.
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