Demyelination in primate autoimmune encephalomyelitis and acute multiple sclerosis lesions: A case for antigen-specific antibody mediation

Cedric S. Raine, Barbara Cannella, Stephen L. Hauser, Claude P. Genain

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

Neuropathological and ultrastructural features of central nervous system demyelination were compared in marmoset experimental autoimmune encephalomyelitis (EAE) induced with myelin/oligodendrocyte glycoprotein (MEG), and in 3 cases of multiple sclerosis (MS) displaying recent lesions. At the edges of EAE and MS lesions, a zone of myelin vacuolation was common, whereas in the lesion proper, myelin sheaths were consistently transformed into vesiculated membranous networks. These networks became dissociated from axons by cell processes from macrophages. Oligodendrocytes were remarkably spared and evidence of myelin repair was present but not prominent. Axonal pathology was more common in the MS material than in marmoset EAE. Immunocytochemistry, using gold-labeled encephalitogenic peptides of MeG and silver enhancement to detect MeG autoantibodies, revealed the presence of MOG-specific autoantibodies over vesiculated myelin networks. Gold-labeled antibody to IgG also gave a positive reaction. Gold-labeled peptide of myelin basic protein did not react with MOG/EAE tissue, but the same conjugate gave positive staining in MS (and in marmoset EAE induced by whole white matter), perhaps indicating broader spectrum immunoreactivity or sensitization to myelin antigens. Thus, vesicular disruption of myelin was a constant feature in these evolving, highly active lesions in primate EAE and MS and appeared causally related to the deposition of antigen-specific autoantibodies.

Original languageEnglish (US)
Pages (from-to)144-160
Number of pages17
JournalAnnals of Neurology
Volume46
Issue number2
DOIs
StatePublished - 1999

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Encephalomyelitis
Autoimmune Experimental Encephalomyelitis
Demyelinating Diseases
Myelin Sheath
Primates
Multiple Sclerosis
Antigens
Callithrix
Antibodies
Gold
Autoantibodies
Myelin-Oligodendrocyte Glycoprotein
Peptides
Myelin Basic Protein
Oligodendroglia
Silver
Axons
Central Nervous System
Immunoglobulin G
Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Demyelination in primate autoimmune encephalomyelitis and acute multiple sclerosis lesions : A case for antigen-specific antibody mediation. / Raine, Cedric S.; Cannella, Barbara; Hauser, Stephen L.; Genain, Claude P.

In: Annals of Neurology, Vol. 46, No. 2, 1999, p. 144-160.

Research output: Contribution to journalArticle

Raine, Cedric S. ; Cannella, Barbara ; Hauser, Stephen L. ; Genain, Claude P. / Demyelination in primate autoimmune encephalomyelitis and acute multiple sclerosis lesions : A case for antigen-specific antibody mediation. In: Annals of Neurology. 1999 ; Vol. 46, No. 2. pp. 144-160.
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