Demonstration of a hematopoietic stem cell antigen (SC‐1) on a murine lymphoma and isolation of variants lacking the antigen

Ross S. Basch, Thomas Panagiotatos, Joan W. Berman, Joel N. Buxbaum

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Murine multipotential hematopoietic stem cells (CFU‐s) bear an antigen (SC‐1) which is recognized by heterologous antisera to mouse brain. We have found that cloned Thy‐1 negative variants of the T‐cell lymphoma RL ♂1 are sensitive to complement‐mediated cytolysis by anti‐brain serum and can absorb the anti‐stem cell activity from the antiserum. We have isolated several subclones derived from a primary Thy‐1 negative variant which are not susceptible to anti‐brain serum. The surface of the resistant lines has little or no antigen capable of binding anti‐mouse brain antibodies as measured by either immunofluorescence or a radioimmunoassay. These lines are also unable to absorb the anti‐bodies responsible for the cytotoxic effect of rabbit anti‐mouse brain serum against CFU‐s. We conclude that the predominant antigen, serologically detectable on Thy‐1 negative variants of RL ♂1, is SC‐1.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalJournal of Cellular Physiology
Volume107
Issue number3
DOIs
StatePublished - Jun 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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