Demonstration of a hematopoietic stem cell antigen (SC-1) on a murine lymphoma and isolation of variants lacking the antigen

R. S. Basch, T. Panagiotatos, Joan W. Berman, J. N. Buxbaum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Murine multipotential hematopoietic stem cells (CFU-s) bear an antigen (SC-1) which is recognized by heterologous antisera to mouse brain. We have found that cloned Thy-1 negative variants of the T-cell lymphoma RLO ♂ 1 are sensitive to complement-mediated cytolysis by anti-brain serum and can absorb the anti-stem cell activity from the antiserum. We have isolated several subclones derived from a primary Thy-1 negative variant which are not susceptible to anti-brain serum. The surface of the resistant lines has little or no antigen capable of binding anti-mouse brain antibodies as measured by either immunofluorescence or a radioimmunoassay. These lines are also unable to absorb the antibodies responsible for the cytotoxic effect of rabbit anti-mouse brain serum against CFU-s. We conclude that the predominant antigen, serologically detectable on Thy-1 negative variants of RL ♂ 1, is SC-1.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalJournal of Cellular Physiology
Volume107
Issue number3
StatePublished - 1981
Externally publishedYes

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Hematopoietic Stem Cells
Stem cells
Lymphoma
Brain
Demonstrations
Antigens
Immune Sera
Serum
T-cells
Antibodies
T-Cell Lymphoma
Radioimmunoassay
Fluorescent Antibody Technique
Stem Cells
Rabbits

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Demonstration of a hematopoietic stem cell antigen (SC-1) on a murine lymphoma and isolation of variants lacking the antigen. / Basch, R. S.; Panagiotatos, T.; Berman, Joan W.; Buxbaum, J. N.

In: Journal of Cellular Physiology, Vol. 107, No. 3, 1981, p. 379-384.

Research output: Contribution to journalArticle

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