Demonstration of a conserved histidine and two water ligands at the Mn2+ site in diocleinae lectins by pulsed EPR spectroscopy

H. Caroline Lee, Alexander K. Goroncy, Jack Peisach, Benildo S. Cavada, Thalles B. Grangeiro, Marcio V. Ramos, Alexandre H. Sampaio, Tarun K. Dam, Curtis F. Brewer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lectins from the Diocleinae subtribe, including Canavalia brasiliensis, Canavalia bonariensis, Canavalia grandiflora, Cratylia floribunda, Dioclea grandiflora, Dioclea guianensis, Dioclea rostrata, Dioclea violacea, and Dioclea virgata, have been recently isolated and characterized in terms of their carbohydrate binding specificities. Although all of the lectins are Man/Glc specific, they possess different biological activities. In the present study, electron paramagnetic resonance (EPR) spectroscopy demonstrates that all nine Diocleinae lectins contain Mn2+. The spectra of C. floribunda and D. rostrata suggest Mn2+ site symmetry different from that of the other seven lectins. However, electron spin-echo envelope modulation (ESEEM) spectroscopy indicates that all nine lectins are coordinated to a histidyl imidazole, with similar electron-nuclear coupling to the Mn2+-bound imidazole nitrogen. ESEEM also demonstrates ligation of two water molecules to Mn2+ in all nine Diocleinae lectins. Thus, the EPR and ESEEM data indicate the presence of a Mn2+ binding site in the above Diocleinae lectins with a conserved histidine residue and two water ligands.

Original languageEnglish (US)
Pages (from-to)2340-2346
Number of pages7
JournalBiochemistry
Volume39
Issue number9
DOIs
StatePublished - Mar 7 2000

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Dioclea
Electron Spin Resonance Spectroscopy
Histidine
Lectins
Paramagnetic resonance
Spectrum Analysis
Demonstrations
Spectroscopy
Ligands
Canavalia
Water
Electrons
Modulation
Bioactivity
Ligation
Nitrogen
Binding Sites
Carbohydrates
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Demonstration of a conserved histidine and two water ligands at the Mn2+ site in diocleinae lectins by pulsed EPR spectroscopy. / Lee, H. Caroline; Goroncy, Alexander K.; Peisach, Jack; Cavada, Benildo S.; Grangeiro, Thalles B.; Ramos, Marcio V.; Sampaio, Alexandre H.; Dam, Tarun K.; Brewer, Curtis F.

In: Biochemistry, Vol. 39, No. 9, 07.03.2000, p. 2340-2346.

Research output: Contribution to journalArticle

Lee, HC, Goroncy, AK, Peisach, J, Cavada, BS, Grangeiro, TB, Ramos, MV, Sampaio, AH, Dam, TK & Brewer, CF 2000, 'Demonstration of a conserved histidine and two water ligands at the Mn2+ site in diocleinae lectins by pulsed EPR spectroscopy', Biochemistry, vol. 39, no. 9, pp. 2340-2346. https://doi.org/10.1021/bi992102b
Lee, H. Caroline ; Goroncy, Alexander K. ; Peisach, Jack ; Cavada, Benildo S. ; Grangeiro, Thalles B. ; Ramos, Marcio V. ; Sampaio, Alexandre H. ; Dam, Tarun K. ; Brewer, Curtis F. / Demonstration of a conserved histidine and two water ligands at the Mn2+ site in diocleinae lectins by pulsed EPR spectroscopy. In: Biochemistry. 2000 ; Vol. 39, No. 9. pp. 2340-2346.
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