TY - JOUR
T1 - Deletion of the last five C-terminal amino acid residues of connexin43 leads to lethal ventricular arrhythmias in mice without affecting coupling via gap junction channels
AU - Lübkemeier, Indra
AU - Requardt, Robert Pascal
AU - Lin, Xianming
AU - Sasse, Philipp
AU - Andrié, René
AU - Schrickel, Jan Wilko
AU - Chkourko, Halina
AU - Bukauskas, Feliksas F.
AU - Kim, Jung Sun
AU - Frank, Marina
AU - Malan, Daniela
AU - Zhang, Jiong
AU - Wirth, Angela
AU - Dobrowolski, Radoslaw
AU - Mohler, Peter J.
AU - Offermanns, Stefan
AU - Fleischmann, Bernd K.
AU - Delmar, Mario
AU - Willecke, Klaus
N1 - Funding Information:
This work was supported by Grants of the German Research Foundation [Wi270/32-1 and SFB 645, B2 to K.W.; FL 276/3-3 to B.K.F. and P.S.], the National Institutes of Health [R01 HL106632-01, R01 GM57691-13 to M.D.; R01NS072238, RO1HL084464 to F.F.B., as well as NIH HL084583, HL083422 to P J M], the Leducq Foundation to M.D., as well as the American Heart Association and the Saving Tiny Hearts Society to P.J.M.I.L. gratefully acknowledges a PhD stipend of the Jürgen-Manchot-Foundation. We thank Melanie Jokwitz and Christine Siegmund for excellent technical assistance and blastocyst injections.
PY - 2013/5
Y1 - 2013/5
N2 - The cardiac intercalated disc harbors mechanical and electrical junctions as well as ion channel complexes mediating propagation of electrical impulses. Cardiac connexin43 (Cx43) co-localizes and interacts with several of the proteins located at intercalated discs in the ventricular myocardium. We have generated conditional Cx43D378stop mice lacking the last five C-terminal amino acid residues, representing a binding motif for zonula occludens protein-1 (ZO-1), and investigated the functional consequences of this mutation on cardiac physiology and morphology. Newborn and adult homozygous Cx43D378stop mice displayed markedly impaired and heterogeneous cardiac electrical activation properties and died from severe ventricular arrhythmias. Cx43 and ZO-1 were co-localized at intercalated discs in Cx43D378stop hearts, and the Cx43D378stop gap junction channels showed normal coupling properties. Patch clamp analyses of isolated adult Cx43D378stop cardiomyocytes revealed a significant decrease in sodium and potassium current densities. Furthermore, we also observed a significant loss of Nav1.5 protein from intercalated discs in Cx43D378stop hearts. The phenotypic lethality of the Cx43D378stop mutation was very similar to the one previously reported for adult Cx43 deficient (Cx43KO) mice. Yet, in contrast to Cx43KO mice, the Cx43 gap junction channel was still functional in the Cx43D378stop mutant. We conclude that the lethality of Cx43D378stop mice is independent of the loss of gap junctional intercellular communication, but most likely results from impaired cardiac sodium and potassium currents. The Cx43D378stop mice reveal for the first time that Cx43 dependent arrhythmias can develop by mechanisms other than impairment of gap junction channel function.
AB - The cardiac intercalated disc harbors mechanical and electrical junctions as well as ion channel complexes mediating propagation of electrical impulses. Cardiac connexin43 (Cx43) co-localizes and interacts with several of the proteins located at intercalated discs in the ventricular myocardium. We have generated conditional Cx43D378stop mice lacking the last five C-terminal amino acid residues, representing a binding motif for zonula occludens protein-1 (ZO-1), and investigated the functional consequences of this mutation on cardiac physiology and morphology. Newborn and adult homozygous Cx43D378stop mice displayed markedly impaired and heterogeneous cardiac electrical activation properties and died from severe ventricular arrhythmias. Cx43 and ZO-1 were co-localized at intercalated discs in Cx43D378stop hearts, and the Cx43D378stop gap junction channels showed normal coupling properties. Patch clamp analyses of isolated adult Cx43D378stop cardiomyocytes revealed a significant decrease in sodium and potassium current densities. Furthermore, we also observed a significant loss of Nav1.5 protein from intercalated discs in Cx43D378stop hearts. The phenotypic lethality of the Cx43D378stop mutation was very similar to the one previously reported for adult Cx43 deficient (Cx43KO) mice. Yet, in contrast to Cx43KO mice, the Cx43 gap junction channel was still functional in the Cx43D378stop mutant. We conclude that the lethality of Cx43D378stop mice is independent of the loss of gap junctional intercellular communication, but most likely results from impaired cardiac sodium and potassium currents. The Cx43D378stop mice reveal for the first time that Cx43 dependent arrhythmias can develop by mechanisms other than impairment of gap junction channel function.
KW - Connexin43
KW - Intercalated disc
KW - Zonula occludens protein-1
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U2 - 10.1007/s00395-013-0348-y
DO - 10.1007/s00395-013-0348-y
M3 - Article
C2 - 23558439
AN - SCOPUS:84875663184
SN - 0300-8428
VL - 108
JO - Basic research in cardiology
JF - Basic research in cardiology
IS - 3
M1 - 348
ER -