Definition of a Large Region of RAG1 That Is Important for Coimmunoprecipitation of RAG2

Catherine J. McMahan, Moshe J. Sadofsky, David G. Schatz

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Interaction between the RAG1 and RAG2 proteins is probably critical for V(D)J recombination. Using a coimmunoprecipitation assay, we define a large region of RAG1 (amino acids 504-1008) that is sufficient for interaction with RAG2. This region comprises the C-terminal half of the RAG1 protein, and is within the region defined as the recombinationally active core. Deletion of either of two regions of RAG1 (amino acids 504-570 or 850-1008) causes a loss of interaction with RAG2. Loss of coimmunoprecipitation is also seen with RAG1 core proteins containing deletions of smaller stretches of amino acids (amino acids 506-511 or 545-550), emphasizing the importance of this region of RAG1 in forming a complex with RAG2. A variety of other small deletion mutations within the amino acid region 504-1008 also decrease coimmunoprecipitation of RAG2 with RAG1, indicating that much or all of this region is important for complex formation.

Original languageEnglish (US)
Pages (from-to)2202-2210
Number of pages9
JournalJournal of Immunology
Volume158
Issue number5
StatePublished - Mar 1 1997
Externally publishedYes

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Amino Acids
V(D)J Recombination
Proteins
Sequence Deletion

ASJC Scopus subject areas

  • Immunology

Cite this

Definition of a Large Region of RAG1 That Is Important for Coimmunoprecipitation of RAG2. / McMahan, Catherine J.; Sadofsky, Moshe J.; Schatz, David G.

In: Journal of Immunology, Vol. 158, No. 5, 01.03.1997, p. 2202-2210.

Research output: Contribution to journalArticle

McMahan, Catherine J. ; Sadofsky, Moshe J. ; Schatz, David G. / Definition of a Large Region of RAG1 That Is Important for Coimmunoprecipitation of RAG2. In: Journal of Immunology. 1997 ; Vol. 158, No. 5. pp. 2202-2210.
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