Defects in hemostasis in P-selectin-deficient mice

Meera Subramaniam, Paul S. Frenette, Simin Saffaripour, Robert C. Johnson, Richard O. Hynes, Denisa D. Wagner

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P- selectin in hemostasis (Frenette et al, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin-deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-α in the P-selectin-deficient mice. The hemorrhagic lesions, quantitated both macroscopically end microscopically, were twofold larger in the P-selectin-deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells. Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling.

Original languageEnglish (US)
Pages (from-to)1238-1242
Number of pages5
JournalBlood
Volume87
Issue number4
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

P-Selectin
Hemostasis
Defects
Platelets
Skin
Blood Platelets
Shwartzman Phenomenon
Bleeding Time
Radioactivity
Chromium
Amputation
Endothelium
Lipopolysaccharides
Tail
Leukocytes
Blood
Tumor Necrosis Factor-alpha
Erythrocytes
Cells
Injections

ASJC Scopus subject areas

  • Hematology

Cite this

Subramaniam, M., Frenette, P. S., Saffaripour, S., Johnson, R. C., Hynes, R. O., & Wagner, D. D. (1996). Defects in hemostasis in P-selectin-deficient mice. Blood, 87(4), 1238-1242.

Defects in hemostasis in P-selectin-deficient mice. / Subramaniam, Meera; Frenette, Paul S.; Saffaripour, Simin; Johnson, Robert C.; Hynes, Richard O.; Wagner, Denisa D.

In: Blood, Vol. 87, No. 4, 15.02.1996, p. 1238-1242.

Research output: Contribution to journalArticle

Subramaniam, M, Frenette, PS, Saffaripour, S, Johnson, RC, Hynes, RO & Wagner, DD 1996, 'Defects in hemostasis in P-selectin-deficient mice', Blood, vol. 87, no. 4, pp. 1238-1242.
Subramaniam M, Frenette PS, Saffaripour S, Johnson RC, Hynes RO, Wagner DD. Defects in hemostasis in P-selectin-deficient mice. Blood. 1996 Feb 15;87(4):1238-1242.
Subramaniam, Meera ; Frenette, Paul S. ; Saffaripour, Simin ; Johnson, Robert C. ; Hynes, Richard O. ; Wagner, Denisa D. / Defects in hemostasis in P-selectin-deficient mice. In: Blood. 1996 ; Vol. 87, No. 4. pp. 1238-1242.
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