Defective Fc-mediated phagocytosis in C3H/HeJ macrophages. II. Correction by cAMP agonists

S. N. Vogel, L. L. Weedon, J. J. Oppenheim, David L. Rosenstreich

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Peritoneal macrophages from LPS hyporesponsive C3H/HeJ mice lose the capacity to bind and phagocytose opsonized sheep erythrocytes (EA) over a 48-hr culture period. this loss in Fc receptor capacity is markedly different from the progressive increase in phagocytic ability exhibited by cultured macrophages derived from LPS-responsive C3H/HeN mice. Since dibutyryl-cyclic adenosine monophosphate (DBcAMP) has previously been reported to modulate membrane receptor expression in lymphocytes and certain macrophage-like cell lines, we examined its effects on EA binding and phagocytosis by C3H/HeJ macrophages. DBcAMP not only reverses the binding defect in C3H/HeJ macrophages but also restores EA phogocytosis to the level of control C3H/HeN cultures. 8-Bromo-cAMP, as well as other agents known to elevate intracellular cAMP (i.e., isoproternol plus isobutylmethylxanthine or prostaglandin E2) also corrected the phagocytic defect. Since the C3H/HeJ macrophage phagocytic defect can also be reversed by in vitro stimulation with a lymphokine-rich culture supernatant, we examined the effect of this treatment on intracellular cAMP levels. Lymphokine treatment produced a 60% increase in the levels of macrophage intracellular cAMP. These findings suggest that the C3H/HeJ differentiation defect may be secondary to some abnormality in a cAMP dependent pathway.

Original languageEnglish (US)
Pages (from-to)441-445
Number of pages5
JournalJournal of Immunology
Volume126
Issue number2
StatePublished - 1981
Externally publishedYes

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Phagocytosis
Macrophages
Inbred C3H Mouse
Lymphokines
Cyclic AMP
8-Bromo Cyclic Adenosine Monophosphate
Fc Receptors
Peritoneal Macrophages
Dinoprostone
Sheep
Erythrocytes
Lymphocytes
Cell Line
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Defective Fc-mediated phagocytosis in C3H/HeJ macrophages. II. Correction by cAMP agonists. / Vogel, S. N.; Weedon, L. L.; Oppenheim, J. J.; Rosenstreich, David L.

In: Journal of Immunology, Vol. 126, No. 2, 1981, p. 441-445.

Research output: Contribution to journalArticle

Vogel, S. N. ; Weedon, L. L. ; Oppenheim, J. J. ; Rosenstreich, David L. / Defective Fc-mediated phagocytosis in C3H/HeJ macrophages. II. Correction by cAMP agonists. In: Journal of Immunology. 1981 ; Vol. 126, No. 2. pp. 441-445.
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