Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein

Olena S. Oliinyk; Chenshuo Ma; Sergei Pletnev; Mikhail Baloban; Carlos Toboada; Huaxin Sheng; Junjie Yao; Vladislav V. Verkhusha

doi:10.1038/s41592-022-01683-0

Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein

Olena S. Oliinyk, Chenshuo Ma, Sergei Pletnev, Mikhail Baloban, Carlos Toboada, Huaxin Sheng, Junjie Yao, Vladislav V. Verkhusha

Genetics

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.

Original language	English (US)
Pages (from-to)	70-74
Number of pages	5
Journal	Nature Methods
Volume	20
Issue number	1
DOIs	https://doi.org/10.1038/s41592-022-01683-0
State	Published - Jan 2023

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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title = "Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein",

abstract = "Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.",

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AU - Oliinyk, Olena S.

AU - Ma, Chenshuo

AU - Pletnev, Sergei

AU - Baloban, Mikhail

AU - Toboada, Carlos

AU - Sheng, Huaxin

AU - Yao, Junjie

AU - Verkhusha, Vladislav V.

PY - 2023/1

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AB - Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.

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Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein

Abstract

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