@article{7e7cf8f4333c49ffa2773efa3514c80e,
title = "Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein",
abstract = "Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.",
author = "Oliinyk, {Olena S.} and Chenshuo Ma and Sergei Pletnev and Mikhail Baloban and Carlos Toboada and Huaxin Sheng and Junjie Yao and Verkhusha, {Vladislav V.}",
note = "Funding Information: We thank N. Peitsaro and N. Aarnio from the Flow Cytometry Core Facility of the University of Helsinki for the technical assistance. This work was supported by grants from the US National Institutes of Health (NIH) (grant nos. GM122567, EB028143, NS111039 and NS115581), Chan Zuckerberg Initiative (grant no. 226178), Cancer Foundation Finland and Magnus Ehrnrooth Foundation. S.P. was supported in part by the NIH Intramural Research Program for the Vaccine Research Center of the National Institute of Allergy and Infectious Diseases. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2023",
month = jan,
doi = "10.1038/s41592-022-01683-0",
language = "English (US)",
volume = "20",
pages = "70--74",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "1",
}