Decreased vaginal disease in J-chain-deficient mice following herpes simplex type 2 genital infection

Barbara A. Hendrickson, Jun Guo, Ian Brown, Kimberly Dennis, Daniel Marcellino, Jill Hetzel, Betsy Herold

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

J-chain-deficient (Jch(-/-)) mice were used to study the role of polymeric IgA (plgA) in primary disease and protective immunity following genital herpes simplex type 2 (HSV-2) infection. Vaginal IgA in the Jch(-/-) mice was composed primarily of monomeric IgA and was not associated with secretory component (SC). In contrast, vaginal IgA in wild-type (WT) mice was predominantly polymeric and bound to SC. Following HSV-2 genital infection, the Jch(-/-) mice consistently exhibited fewer vaginal symptoms (P = 0.010) and mortality (P = 0.075) than did the WT mice. The variation in disease expression could not be explained by differences in local viral replication, since titers in vaginal wash fluid were comparable. To assess the effect of J chain deficiency on protective immunity, WT and Jch(-/-) mice were immunized intravaginally with attenuated HSV-2, challenged intravaginally with wild- type virus 5 weeks later, and evaluated for vaginal infection and neurological disease. Although the Jch(-/-) mice had reduced vaginal HSV- specific IgA and IgG levels following immunization, both WT and Jch(-/-) mice were protected from symptoms following wild-type virus challenge. We conclude that plgA is not required for protective immunity against genital HSV-2 disease and that J chain deficiency offers some protection against symptoms following primary HSV-2 genital infection.

Original languageEnglish (US)
Pages (from-to)155-162
Number of pages8
JournalVirology
Volume271
Issue number1
DOIs
StatePublished - May 25 2000
Externally publishedYes

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Vaginal Diseases
Herpes Genitalis
Human Herpesvirus 2
Infection
Immunoglobulin A
Secretory Component
Immunity
Viruses
Immunization
Immunoglobulin G

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Hendrickson, B. A., Guo, J., Brown, I., Dennis, K., Marcellino, D., Hetzel, J., & Herold, B. (2000). Decreased vaginal disease in J-chain-deficient mice following herpes simplex type 2 genital infection. Virology, 271(1), 155-162. https://doi.org/10.1006/viro.2000.0303

Decreased vaginal disease in J-chain-deficient mice following herpes simplex type 2 genital infection. / Hendrickson, Barbara A.; Guo, Jun; Brown, Ian; Dennis, Kimberly; Marcellino, Daniel; Hetzel, Jill; Herold, Betsy.

In: Virology, Vol. 271, No. 1, 25.05.2000, p. 155-162.

Research output: Contribution to journalArticle

Hendrickson, BA, Guo, J, Brown, I, Dennis, K, Marcellino, D, Hetzel, J & Herold, B 2000, 'Decreased vaginal disease in J-chain-deficient mice following herpes simplex type 2 genital infection', Virology, vol. 271, no. 1, pp. 155-162. https://doi.org/10.1006/viro.2000.0303
Hendrickson BA, Guo J, Brown I, Dennis K, Marcellino D, Hetzel J et al. Decreased vaginal disease in J-chain-deficient mice following herpes simplex type 2 genital infection. Virology. 2000 May 25;271(1):155-162. https://doi.org/10.1006/viro.2000.0303
Hendrickson, Barbara A. ; Guo, Jun ; Brown, Ian ; Dennis, Kimberly ; Marcellino, Daniel ; Hetzel, Jill ; Herold, Betsy. / Decreased vaginal disease in J-chain-deficient mice following herpes simplex type 2 genital infection. In: Virology. 2000 ; Vol. 271, No. 1. pp. 155-162.
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