Decreased gap-junctional communication associated with segregation of the neuronal phenotype in the RT4 cell-line family

Laurel M. Donahue, Daniel R. Webster, Ivvanee Martinez, David C. Spray

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

RT4 is a family of cell lines derived from a rat peripheral neurotumor. The RT4 family consists of a multipotential stem-cell line that spontaneously gives rise to three derivative cell types, one glial and two neuronal. The three derivative cell types are capable of further lineage-specific maturation under appropriate culture conditions. Gap-junctional communication is postulated to be important during nervous-system development by allowing and/or controlling the transmission of both electrical current and signaling molecules, which may affect growth and differentiation. Our characterization of gap-junctional communication in the RT4 cell line family revealed that: (1) the glial-derivative and the stem-cell line were extensively coupled, while the two neuronal derivatives were significantly less coupled, and (2) all of the RT4 cell lines, including the stem-cell line, expressed Cx43 mRNA and protein, and the levels were generally consistent with the observed degree of functional coupling. These observations are consistent with data from in vivo studies and establish the RT4 cell line family as a potentially useful in vitro model system for understanding the role(s) of gap-junctional communication during differentiation in the peripheral nervous system.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalCell and Tissue Research
Volume292
Issue number1
DOIs
StatePublished - 1998

Fingerprint

Stem cells
Communication
Cells
Derivatives
Phenotype
Cell Line
Neurology
Stem Cells
Connexin 43
Neuroglia
Rats
Peripheral Nervous System
Messenger RNA
Molecules
Nervous System
Proteins
Growth

Keywords

  • Connexin
  • Cx43
  • Differentiation
  • Neural crest
  • Neural development
  • Peripheral nervous system
  • Stem cell

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Decreased gap-junctional communication associated with segregation of the neuronal phenotype in the RT4 cell-line family. / Donahue, Laurel M.; Webster, Daniel R.; Martinez, Ivvanee; Spray, David C.

In: Cell and Tissue Research, Vol. 292, No. 1, 1998, p. 27-35.

Research output: Contribution to journalArticle

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AU - Spray, David C.

PY - 1998

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