Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease

Michael E.T.I. Boerrigter, Cornelia M. Van Duijn, Erik Mullaart, Piet Eikelenboom, Cees M.A. Van Der Togt, Dick L. Knook, Albert Hofman, Jan Vijg

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 ± 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 ± 8.2% and 43.0 ± 4.4%, respectively; p>0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 ± 5.8%, which was significantly lower than that found in controls (p<0.01) or in the other AD patients (p<0.02).

Original languageEnglish (US)
Pages (from-to)367-370
Number of pages4
JournalNeurobiology of Aging
Volume12
Issue number4
DOIs
StatePublished - 1991

Keywords

  • Alkaline elution
  • Alzheimer
  • DNA repair
  • Ethylnitrosourea
  • Lymphocytes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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    Boerrigter, M. E. T. I., Van Duijn, C. M., Mullaart, E., Eikelenboom, P., Van Der Togt, C. M. A., Knook, D. L., Hofman, A., & Vijg, J. (1991). Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease. Neurobiology of Aging, 12(4), 367-370. https://doi.org/10.1016/0197-4580(91)90024-E