De novo synaptogenesis induced by GABA in the developing mouse cortex

Won Chan Oh, Stefano Lutzu, Pablo E. Castillo, Hyung Bae Kwon

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Dendrites of cortical pyramidal neurons contain intermingled excitatory and inhibitory synapses. We studied the local mechanisms that regulate the formation and distribution of synapses. We found that local γ-aminobutyric acid (GABA) release on dendrites of mouse cortical layer 2/3 pyramidal neurons could induce gephyrin puncta and dendritic spine formation via GABA type A receptor activation and voltage-gated calcium channels during early postnatal development. Furthermore, the newly formed inhibitory and excitatory synaptic structures rapidly gained functions. Bidirectional manipulation of GABA release from somatostatin-positive interneurons increased and decreased the number of gephyrin puncta and dendritic spines, respectively. These results highlight a noncanonical function of GABA as a local synaptogenic element shaping the early establishment of neuronal circuitry in mouse cortex.

Original languageEnglish (US)
Pages (from-to)1037-1040
Number of pages4
JournalScience
Volume353
Issue number6303
DOIs
StatePublished - Sep 2 2016

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gamma-Aminobutyric Acid
Dendritic Spines
Pyramidal Cells
Dendrites
Synapses
Aminobutyrates
Interneurons
GABA-A Receptors
Calcium Channels
Somatostatin
gephyrin

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

De novo synaptogenesis induced by GABA in the developing mouse cortex. / Oh, Won Chan; Lutzu, Stefano; Castillo, Pablo E.; Kwon, Hyung Bae.

In: Science, Vol. 353, No. 6303, 02.09.2016, p. 1037-1040.

Research output: Contribution to journalArticle

Oh, Won Chan ; Lutzu, Stefano ; Castillo, Pablo E. ; Kwon, Hyung Bae. / De novo synaptogenesis induced by GABA in the developing mouse cortex. In: Science. 2016 ; Vol. 353, No. 6303. pp. 1037-1040.
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