Cytotoxicity by matrix metalloprotease-1 in organotypic spinal cord and dissociated neuronal cultures

Catharina M.P. Vos, Lucas Sjulson, Avindra Nath, Justin C. McArthur, Carlos A. Pardo, Jeffrey Rothstein, Katherine Conant

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Extracellular matrix (ECM) proteins, including collagens and laminins, are critical to the structure of the neuronal synapse and may also be involved in cell survival. In the present study, we therefore examined the possibility that select ECM degrading proteins might be toxic to organotypic spinal cord and dissociated neuronal cultures. Of those proteins tested, including MMP-1, -7, and -9, we observed that MMP-1 was toxic to spinal cord cultures as determined by release of lactic acid dehydrogenase as well as uptake of propidium iodide. Pretreatment of cell cultures with 50 μM α- tocopherol partially reversed these effects. We also observed that MMP-1 was toxic to human neurons grown in dissociated cultures and that increased amounts of MMP-1 were released by astrocytes following their stimulation with IL-1β. These results suggest that further studies may be warranted to determine whether MMP-1 contributes to neurodegenerative conditions in which activated astrocytes may play a role. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalExperimental Neurology
Volume163
Issue number2
DOIs
StatePublished - Jun 2000
Externally publishedYes

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Fingerprint Dive into the research topics of 'Cytotoxicity by matrix metalloprotease-1 in organotypic spinal cord and dissociated neuronal cultures'. Together they form a unique fingerprint.

  • Cite this