Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin

Iryna Berezniuk, Peter J. Lyons, Juan J. Sironi, Hui Xiao, Mitsutoshi Setou, Ruth H. Angeletti, Koji Ikegami, Lloyd D. Fricker

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Cytosolic carboxypeptidase 5 (CCP5) was proposed to selectively cleave γ-linked glutamate from tubulin side chains, but not α-linked glutamates. Results: Purified CCP5 removes α- and γ-linked glutamates from microtubules, soluble tubulin, and synthetic peptides. Conclusion: CCP5 is not specific for γ-linked glutamate or for a specific form of tubulin. Significance: CCP5 is an important tubulin code-modifying enzyme.

Original languageEnglish (US)
Pages (from-to)30445-30453
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
StatePublished - Oct 18 2013

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Glutamates
Carboxypeptidases
Tubulin
Glutamic Acid
Microtubules
Peptides
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Berezniuk, I., Lyons, P. J., Sironi, J. J., Xiao, H., Setou, M., Angeletti, R. H., ... Fricker, L. D. (2013). Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin. Journal of Biological Chemistry, 288(42), 30445-30453. https://doi.org/10.1074/jbc.M113.497917

Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin. / Berezniuk, Iryna; Lyons, Peter J.; Sironi, Juan J.; Xiao, Hui; Setou, Mitsutoshi; Angeletti, Ruth H.; Ikegami, Koji; Fricker, Lloyd D.

In: Journal of Biological Chemistry, Vol. 288, No. 42, 18.10.2013, p. 30445-30453.

Research output: Contribution to journalArticle

Berezniuk, I, Lyons, PJ, Sironi, JJ, Xiao, H, Setou, M, Angeletti, RH, Ikegami, K & Fricker, LD 2013, 'Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin', Journal of Biological Chemistry, vol. 288, no. 42, pp. 30445-30453. https://doi.org/10.1074/jbc.M113.497917
Berezniuk I, Lyons PJ, Sironi JJ, Xiao H, Setou M, Angeletti RH et al. Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin. Journal of Biological Chemistry. 2013 Oct 18;288(42):30445-30453. https://doi.org/10.1074/jbc.M113.497917
Berezniuk, Iryna ; Lyons, Peter J. ; Sironi, Juan J. ; Xiao, Hui ; Setou, Mitsutoshi ; Angeletti, Ruth H. ; Ikegami, Koji ; Fricker, Lloyd D. / Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 42. pp. 30445-30453.
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