Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin

Iryna Berezniuk, Peter J. Lyons, Juan J. Sironi, Hui Xiao, Mitsutoshi Setou, Ruth H. Angeletti, Koji Ikegami, Lloyd D. Fricker

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21 Scopus citations

Abstract

Background: Cytosolic carboxypeptidase 5 (CCP5) was proposed to selectively cleave γ-linked glutamate from tubulin side chains, but not α-linked glutamates. Results: Purified CCP5 removes α- and γ-linked glutamates from microtubules, soluble tubulin, and synthetic peptides. Conclusion: CCP5 is not specific for γ-linked glutamate or for a specific form of tubulin. Significance: CCP5 is an important tubulin code-modifying enzyme.

Original languageEnglish (US)
Pages (from-to)30445-30453
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
Publication statusPublished - Oct 18 2013

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Berezniuk, I., Lyons, P. J., Sironi, J. J., Xiao, H., Setou, M., Angeletti, R. H., ... Fricker, L. D. (2013). Cytosolic carboxypeptidase 5 removes α- And γ-linked glutamates from tubulin. Journal of Biological Chemistry, 288(42), 30445-30453. https://doi.org/10.1074/jbc.M113.497917