Cytokine-induced programmed death of cultured sympathetic neurons

John A. Kessler, William H. Ludlam, Mona M. Freidin, David H. Hall, Marc D. Michaelson, David C. Spray, Maryjane Dougherty, David K. Batter

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Programmed cell death (PCD) of sympathetic neurons is inhibited by nerve growth factor. However, factors that induce PCD of these cells are unknown. Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor, neuropoietic cytokines known to regulate sympathetic neuron gene expression, were examined for effects on survival of cultured sympathetic neurons. Treatment with LIF or ciliary neurotrophic factor caused neuronal death in a dose-dependent fashion. Inhibition of RNA or protein synthesis, or treatment with potassium, all of which prevent PCD after nerve growth factor deprivation, prevented LIF-induced death. The morphologic and ultrastructural characteristics of the neuronal death induced by LIF and by nerve growth factor deprivation were similar. Furthermore, LIF treatment resulted in DNA fragmentation with a characteristic "ladder" on Southern blot analysis. These observations suggest that neuron numbers may be regulated by factors which initiate PCD, as well as by factors which prevent it.

Original languageEnglish (US)
Pages (from-to)1123-1132
Number of pages10
JournalNeuron
Volume11
Issue number6
DOIs
StatePublished - 1993

Fingerprint

Leukemia Inhibitory Factor
Cytokines
Neurons
Nerve Growth Factor
Cell Death
Ciliary Neurotrophic Factor
DNA Fragmentation
Southern Blotting
Potassium
RNA
Gene Expression
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kessler, J. A., Ludlam, W. H., Freidin, M. M., Hall, D. H., Michaelson, M. D., Spray, D. C., ... Batter, D. K. (1993). Cytokine-induced programmed death of cultured sympathetic neurons. Neuron, 11(6), 1123-1132. https://doi.org/10.1016/0896-6273(93)90225-G

Cytokine-induced programmed death of cultured sympathetic neurons. / Kessler, John A.; Ludlam, William H.; Freidin, Mona M.; Hall, David H.; Michaelson, Marc D.; Spray, David C.; Dougherty, Maryjane; Batter, David K.

In: Neuron, Vol. 11, No. 6, 1993, p. 1123-1132.

Research output: Contribution to journalArticle

Kessler, JA, Ludlam, WH, Freidin, MM, Hall, DH, Michaelson, MD, Spray, DC, Dougherty, M & Batter, DK 1993, 'Cytokine-induced programmed death of cultured sympathetic neurons', Neuron, vol. 11, no. 6, pp. 1123-1132. https://doi.org/10.1016/0896-6273(93)90225-G
Kessler, John A. ; Ludlam, William H. ; Freidin, Mona M. ; Hall, David H. ; Michaelson, Marc D. ; Spray, David C. ; Dougherty, Maryjane ; Batter, David K. / Cytokine-induced programmed death of cultured sympathetic neurons. In: Neuron. 1993 ; Vol. 11, No. 6. pp. 1123-1132.
@article{5e47a483bdab466fad647144f9539864,
title = "Cytokine-induced programmed death of cultured sympathetic neurons",
abstract = "Programmed cell death (PCD) of sympathetic neurons is inhibited by nerve growth factor. However, factors that induce PCD of these cells are unknown. Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor, neuropoietic cytokines known to regulate sympathetic neuron gene expression, were examined for effects on survival of cultured sympathetic neurons. Treatment with LIF or ciliary neurotrophic factor caused neuronal death in a dose-dependent fashion. Inhibition of RNA or protein synthesis, or treatment with potassium, all of which prevent PCD after nerve growth factor deprivation, prevented LIF-induced death. The morphologic and ultrastructural characteristics of the neuronal death induced by LIF and by nerve growth factor deprivation were similar. Furthermore, LIF treatment resulted in DNA fragmentation with a characteristic {"}ladder{"} on Southern blot analysis. These observations suggest that neuron numbers may be regulated by factors which initiate PCD, as well as by factors which prevent it.",
author = "Kessler, {John A.} and Ludlam, {William H.} and Freidin, {Mona M.} and Hall, {David H.} and Michaelson, {Marc D.} and Spray, {David C.} and Maryjane Dougherty and Batter, {David K.}",
year = "1993",
doi = "10.1016/0896-6273(93)90225-G",
language = "English (US)",
volume = "11",
pages = "1123--1132",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - Cytokine-induced programmed death of cultured sympathetic neurons

AU - Kessler, John A.

AU - Ludlam, William H.

AU - Freidin, Mona M.

AU - Hall, David H.

AU - Michaelson, Marc D.

AU - Spray, David C.

AU - Dougherty, Maryjane

AU - Batter, David K.

PY - 1993

Y1 - 1993

N2 - Programmed cell death (PCD) of sympathetic neurons is inhibited by nerve growth factor. However, factors that induce PCD of these cells are unknown. Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor, neuropoietic cytokines known to regulate sympathetic neuron gene expression, were examined for effects on survival of cultured sympathetic neurons. Treatment with LIF or ciliary neurotrophic factor caused neuronal death in a dose-dependent fashion. Inhibition of RNA or protein synthesis, or treatment with potassium, all of which prevent PCD after nerve growth factor deprivation, prevented LIF-induced death. The morphologic and ultrastructural characteristics of the neuronal death induced by LIF and by nerve growth factor deprivation were similar. Furthermore, LIF treatment resulted in DNA fragmentation with a characteristic "ladder" on Southern blot analysis. These observations suggest that neuron numbers may be regulated by factors which initiate PCD, as well as by factors which prevent it.

AB - Programmed cell death (PCD) of sympathetic neurons is inhibited by nerve growth factor. However, factors that induce PCD of these cells are unknown. Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor, neuropoietic cytokines known to regulate sympathetic neuron gene expression, were examined for effects on survival of cultured sympathetic neurons. Treatment with LIF or ciliary neurotrophic factor caused neuronal death in a dose-dependent fashion. Inhibition of RNA or protein synthesis, or treatment with potassium, all of which prevent PCD after nerve growth factor deprivation, prevented LIF-induced death. The morphologic and ultrastructural characteristics of the neuronal death induced by LIF and by nerve growth factor deprivation were similar. Furthermore, LIF treatment resulted in DNA fragmentation with a characteristic "ladder" on Southern blot analysis. These observations suggest that neuron numbers may be regulated by factors which initiate PCD, as well as by factors which prevent it.

UR - http://www.scopus.com/inward/record.url?scp=0027742346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027742346&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(93)90225-G

DO - 10.1016/0896-6273(93)90225-G

M3 - Article

C2 - 8274279

AN - SCOPUS:0027742346

VL - 11

SP - 1123

EP - 1132

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 6

ER -