Cytokine-induced expression of CD1b molecules by peripheral blood monocytes: Influence of 3'-azido-3'-deoxythymidine

A. Giuliani, L. Tentori, R. Pepponi, Steven A. Porcelli, A. Aquino, L. Orlando, M. Sugita, M. B. Brenner, E. Bonmassar, G. Graziani

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

CD1b is a nonpolymorphic, MHC-like molecule, capable of presenting non-peptide antigens (Ags) to CD3+, CD4-, CD8-, αβ or γδT lymphocytes. Previous studies have shown that CD1b can be induced in monocytes/macrophages by GM-CSF+IL-4, and can restrict their presentation of Mycobacterium tuberculosis antigen (Ag) to Ag-specific T cells. Since a number of HIV-positive subjects undergo mycobacterial infections, preliminary studies have been performed to explore whether anti-HIV chemotherapy would influence cytokine-induced CD1b expression in peripheral blood monocytes. The results obtained by treating monocytes with GM-CSF+IL-4, in presence or absence of 3'-azido-3'-deoxythymidine (AZT) showed that: (a) the majority of adherent mononuclear cells (AMNC) collected from peripheral blood of healthy donors, express CD1b molecule on the cell membrane, upon treatment with GMCSF+IL-4; (b) CD1b appearance is mainly due to the de novo induction of CD1b gene expression (as confirmed by Northern blot analysis), rather than to migration of the molecule from the cytoplasm to the plasma membrane (as suggested by Western blot analysis); (c) AZT does not alter the percentage of CD1b+ AMNC treated with the cytokines; (d) however, AZT inhibits cytokine-induced proliferation of AMNC, thus reducing the overall Ag-presenting potential of the host. Our results suggest that the anti-proliferative effect of AZT could depress anti-mycobacteria immunity in AZT-treated subjects, which may have important implication for the clinical outcome of patients harbouring inadequately treated mycobacterial infections.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalPharmacological Research
Volume35
Issue number2
DOIs
StatePublished - Feb 1997
Externally publishedYes

Fingerprint

Zidovudine
Interleukin-4
Monocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Cell Membrane
HIV
CD3 Antigens
T-Lymphocytes
Antigens
Mycobacterium
Infection
Blood Donors
Northern Blotting
Immunity
Cytoplasm
Western Blotting
Macrophages
Gene Expression
Drug Therapy

Keywords

  • AZT
  • CD1
  • CD1b
  • Monocytes
  • Tuberculosis

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cytokine-induced expression of CD1b molecules by peripheral blood monocytes : Influence of 3'-azido-3'-deoxythymidine. / Giuliani, A.; Tentori, L.; Pepponi, R.; Porcelli, Steven A.; Aquino, A.; Orlando, L.; Sugita, M.; Brenner, M. B.; Bonmassar, E.; Graziani, G.

In: Pharmacological Research, Vol. 35, No. 2, 02.1997, p. 135-140.

Research output: Contribution to journalArticle

Giuliani, A, Tentori, L, Pepponi, R, Porcelli, SA, Aquino, A, Orlando, L, Sugita, M, Brenner, MB, Bonmassar, E & Graziani, G 1997, 'Cytokine-induced expression of CD1b molecules by peripheral blood monocytes: Influence of 3'-azido-3'-deoxythymidine', Pharmacological Research, vol. 35, no. 2, pp. 135-140. https://doi.org/10.1006/phrs.1997.0130
Giuliani, A. ; Tentori, L. ; Pepponi, R. ; Porcelli, Steven A. ; Aquino, A. ; Orlando, L. ; Sugita, M. ; Brenner, M. B. ; Bonmassar, E. ; Graziani, G. / Cytokine-induced expression of CD1b molecules by peripheral blood monocytes : Influence of 3'-azido-3'-deoxythymidine. In: Pharmacological Research. 1997 ; Vol. 35, No. 2. pp. 135-140.
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