Cytogenetics of small cell carcinoma of the lung

Doris H. Wurster-Hill, Linda A. Cannizzaro, Olive S. Pettengill, George D. Sorenson, Charles C. Cate, L. Herbert Maurer

Research output: Contribution to journalArticle

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Abstract

Nineteen cell lines derived from various malignant tissues of 15 patients with small cell carcinoma of the lung (SCCL) have been studied. The results showed heterogeneity in all cell lines, with no one consistent abnormality among them. Cell lines from 11 of the patients had minute and double minute chromosomes, and cell lines from 2 patients had abnormally banding regions, designated as ABRs, as distinguished from homogenously staining regions (HSRs). The latter 2 and several of the former cell lines were derived from specimens taken before the patients were placed on therapy. All but 2 of the cell lines had a constant marker load, consisting of 24%-35% of the complement. Some markers remained stable through months and years of culture life, while other markers came and went. Chromosomes #1, #6 and #11 were most frequently involved in market formation in the cell lines, and these were compared to similar markers in direct bone marrow preparations. Chromosome #1 markers were of variable structure, whereas #6 and #11 most often took the form of 6q- and 11p+markers, with breakpoints most frequently at 6q23 - 25 and 11p11 - 12. A 3p- marker was found in a minority of cell lines. All of these markers were also found in direct marrow preparations from some patients with SCCL. Nonmonoclonal tumors arose from inoculation of bimodal cell lines into nude mice, but population selection by undetermined mechanism was evident. Cytogenetic parameters showed no positive correlation with hormone production by these cell lines.

Original languageEnglish (US)
Pages (from-to)303-330
Number of pages28
JournalCancer Genetics and Cytogenetics
Volume13
Issue number4
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Small Cell Lung Carcinoma
Cytogenetics
Cell Line
Chromosomes, Human, Pair 1
Bone Marrow
Chromosomes, Human, Pair 6
Genetic Markers
Nude Mice
Chromosomes
Hormones
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Wurster-Hill, D. H., Cannizzaro, L. A., Pettengill, O. S., Sorenson, G. D., Cate, C. C., & Maurer, L. H. (1984). Cytogenetics of small cell carcinoma of the lung. Cancer Genetics and Cytogenetics, 13(4), 303-330. https://doi.org/10.1016/0165-4608(84)90075-X

Cytogenetics of small cell carcinoma of the lung. / Wurster-Hill, Doris H.; Cannizzaro, Linda A.; Pettengill, Olive S.; Sorenson, George D.; Cate, Charles C.; Maurer, L. Herbert.

In: Cancer Genetics and Cytogenetics, Vol. 13, No. 4, 1984, p. 303-330.

Research output: Contribution to journalArticle

Wurster-Hill, DH, Cannizzaro, LA, Pettengill, OS, Sorenson, GD, Cate, CC & Maurer, LH 1984, 'Cytogenetics of small cell carcinoma of the lung', Cancer Genetics and Cytogenetics, vol. 13, no. 4, pp. 303-330. https://doi.org/10.1016/0165-4608(84)90075-X
Wurster-Hill DH, Cannizzaro LA, Pettengill OS, Sorenson GD, Cate CC, Maurer LH. Cytogenetics of small cell carcinoma of the lung. Cancer Genetics and Cytogenetics. 1984;13(4):303-330. https://doi.org/10.1016/0165-4608(84)90075-X
Wurster-Hill, Doris H. ; Cannizzaro, Linda A. ; Pettengill, Olive S. ; Sorenson, George D. ; Cate, Charles C. ; Maurer, L. Herbert. / Cytogenetics of small cell carcinoma of the lung. In: Cancer Genetics and Cytogenetics. 1984 ; Vol. 13, No. 4. pp. 303-330.
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