Abstract
Conventional (whole body) CYP2E1 knockout mice displayed protection against high-fat dietinduced weight gain, obesity, and hyperlipidemia with increased energy expenditure despite normal food intake and spontaneous locomotor activity. In addition, the CYP2E1 knockout mice displayed a marked improvement in glucose tolerance on both normal chow and high-fat diets. Euglycemic-hyperinsulinemic clamps demonstrated a marked protection against high-fat diet-induced insulin resistance in CYP2E1 knockout mice, with enhanced adipose tissue glucose uptake and insulin suppression of hepatic glucose output. In parallel, adipose tissue was protected against high-fat diet-induced proinflammatory cytokine production. Taken together, these data demonstrate that the CYP2E1 deletion protects mice against high-fat diet-induced insulin resistance with improved glucose homeostasis in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | E532-E539 |
| Journal | American Journal of Physiology - Endocrinology and Metabolism |
| Volume | 302 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 2012 |
Keywords
- Energy expenditure
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Physiology
- Physiology (medical)
