Cystic canal mutants in Caenorhabditis elegans are defective in the apical membrane domain of the renal (excretory) cell

Matthew Buechner, David H. Hall, Harshida Bhatt, Edward M. Hedgecock

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The excretory cell extends a tubular process, or canal, along the basolateral surface of the epidermis to form the nematode renal epithelium. This cell can undergo normal tubulogenesis in isolated cell culture. Mutations in 12 genes cause excretory canal cysts in Caenorhabditis elegans. Genetic interactions, and their similar phenotypes, suggest these genes may encode functionally related proteins. Depending upon genotype and individual canal, defects range from focal cysts, flanked by normal width segments, to regional cysts involving the entire tubule. Oftentimes the enlarged regions are convoluted or partially septated. In mutants with very large cysts, renal function is measurably impaired. Based on histology and ultrastructure, canal cysts likely result from defects of the apical membrane domain. These mutants provide a model of tubulocystic disease without hyperplasia or basement membrane abnormalities. Similar apical mechanisms could regulate tubular morphology of vertebrate nephrons.

Original languageEnglish (US)
Pages (from-to)227-241
Number of pages15
JournalDevelopmental Biology
Volume214
Issue number1
DOIs
StatePublished - Oct 1 1999

Fingerprint

Caenorhabditis elegans
Cysts
Kidney
Membranes
Nephrons
Basement Membrane
Epidermis
Genes
Hyperplasia
Vertebrates
Histology
Epithelium
Cell Culture Techniques
Genotype
Phenotype
Mutation
Proteins

Keywords

  • Excretory canals
  • Nematode
  • Polycystic kidney disease
  • Renal epithelium
  • Tubulogenesis

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Cystic canal mutants in Caenorhabditis elegans are defective in the apical membrane domain of the renal (excretory) cell. / Buechner, Matthew; Hall, David H.; Bhatt, Harshida; Hedgecock, Edward M.

In: Developmental Biology, Vol. 214, No. 1, 01.10.1999, p. 227-241.

Research output: Contribution to journalArticle

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