Cysteine biosynthetic enzymes are the pieces of a metabolic energy pump

Jiang Wei, Qing Xiu Tang, Olga Varlamova, Camille Roche, Reaching Lee, Thomas S. Leyh

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred at the molecular level. These systems catalyze energy transfer through structural change and appear to be dedicated exclusively to their coupling tasks (7, 8). Here we report the discovery of a new class of energy-transfer system. It is a biosynthetic pump composed of cysteine biosynthesis enzymes, ATP sulfurylase and O-acetylserine sulfhydrylase, each with its own catalytic function and from whose interactions emerge new function: the hydrolysis of ATP. The hydrolysis is kinetically and energetically linked to the chemistry catalyzed by ATP sulfurylase, the first enzyme in the cysteine biosynthetic pathway, in such a way that each molecule of ATP hydrolyzed, each stroke of the pump, produces 1 equivalent of that enzyme's product. These findings integrate cysteine metabolism and broaden our understanding of the ways in which higher order allostery is used to effect free energy transfer.

Original languageEnglish (US)
Pages (from-to)8493-8498
Number of pages6
JournalBiochemistry
Volume41
Issue number26
DOIs
StatePublished - Jul 2 2002

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Membrane Transport Proteins
Energy Transfer
Energy transfer
Cysteine
Sulfate Adenylyltransferase
Pumps
Adenosine Triphosphate
Enzymes
Metabolism
Free energy
Hydrolysis
Cysteine Synthase
Chemical potential
Biosynthesis
Biosynthetic Pathways
Protons
Stroke
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cysteine biosynthetic enzymes are the pieces of a metabolic energy pump. / Wei, Jiang; Tang, Qing Xiu; Varlamova, Olga; Roche, Camille; Lee, Reaching; Leyh, Thomas S.

In: Biochemistry, Vol. 41, No. 26, 02.07.2002, p. 8493-8498.

Research output: Contribution to journalArticle

Wei, J, Tang, QX, Varlamova, O, Roche, C, Lee, R & Leyh, TS 2002, 'Cysteine biosynthetic enzymes are the pieces of a metabolic energy pump', Biochemistry, vol. 41, no. 26, pp. 8493-8498. https://doi.org/10.1021/bi025953j
Wei, Jiang ; Tang, Qing Xiu ; Varlamova, Olga ; Roche, Camille ; Lee, Reaching ; Leyh, Thomas S. / Cysteine biosynthetic enzymes are the pieces of a metabolic energy pump. In: Biochemistry. 2002 ; Vol. 41, No. 26. pp. 8493-8498.
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