TY - JOUR
T1 - Cys-93-ββ-succinimidophenyl polyethylene glycol 2000 hemoglobin A
T2 - Intramolecular cross-bridging of hemoglobin outside the central cavity
AU - Manjula, Belur N.
AU - Malavallin, Ashok
AU - Smith, Paul K.
AU - Chan, Nei Li
AU - Arnone, Arthur
AU - Friedman, Joel M.
AU - Acharya, A. Seetharama
PY - 2000/2/25
Y1 - 2000/2/25
N2 - Bis(maleidophenyl)-PEG2000 (Bis-Mal-PEG2000), a new bifunctional protein cross-linker targeted to sulfhydryl groups, introduces intra-tetrameric cross-links into oxy-HbA in nearly quantitative yields. Structural as well as crystallographic analyses of the cross-linked species, Bis-Mal-PEG2000 HbA, identified Cys-93(β) as the site of intramolecular cross-linking. The cross- bridging had only a limited influence on the O2 affinity and cooperativity of HbA in 50 mM BisTris acetate, pH 7.4. However, the Bohr effect was reduced by ~60%. Bis-Mal-PEG2000 HbA retained sensitivity to the presence of allosteric effectors 2,3-diphosphoglycerate, IHP, and chloride, albeit to a lesser degree compared with HbA. Crystallographic analysis revealed the overall structure of deoxy-Bis-Mal-PEG2000 HbA to be similar to deoxy-HbA but for the loss of the salt bridge between Asp-94(β) and His-146(β). The large influence of the cross-bridging on the alkaline Bohr effect of HbA is consistent with the loss of this salt bridge. Unlike the 'central cavity cross-bridges' described previously, the cross-link introduced by Bis-Mal- PEG2000 into HbA is an 'outside the central cavity cross-bridge'. In view of its oxy-conformational specificity and limited influence on O2 affinity, this new cross-linking strategy holds promise for the stabilization of new designer low O2 affinity Hbs generated by recombinant DNA technology for applications as Hb based therapeutics.
AB - Bis(maleidophenyl)-PEG2000 (Bis-Mal-PEG2000), a new bifunctional protein cross-linker targeted to sulfhydryl groups, introduces intra-tetrameric cross-links into oxy-HbA in nearly quantitative yields. Structural as well as crystallographic analyses of the cross-linked species, Bis-Mal-PEG2000 HbA, identified Cys-93(β) as the site of intramolecular cross-linking. The cross- bridging had only a limited influence on the O2 affinity and cooperativity of HbA in 50 mM BisTris acetate, pH 7.4. However, the Bohr effect was reduced by ~60%. Bis-Mal-PEG2000 HbA retained sensitivity to the presence of allosteric effectors 2,3-diphosphoglycerate, IHP, and chloride, albeit to a lesser degree compared with HbA. Crystallographic analysis revealed the overall structure of deoxy-Bis-Mal-PEG2000 HbA to be similar to deoxy-HbA but for the loss of the salt bridge between Asp-94(β) and His-146(β). The large influence of the cross-bridging on the alkaline Bohr effect of HbA is consistent with the loss of this salt bridge. Unlike the 'central cavity cross-bridges' described previously, the cross-link introduced by Bis-Mal- PEG2000 into HbA is an 'outside the central cavity cross-bridge'. In view of its oxy-conformational specificity and limited influence on O2 affinity, this new cross-linking strategy holds promise for the stabilization of new designer low O2 affinity Hbs generated by recombinant DNA technology for applications as Hb based therapeutics.
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U2 - 10.1074/jbc.275.8.5527
DO - 10.1074/jbc.275.8.5527
M3 - Article
C2 - 10681532
AN - SCOPUS:0033621891
SN - 0021-9258
VL - 275
SP - 5527
EP - 5534
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -