Cyclosporine nephrotoxicity: Sodium excretion, autoregulation, and angiotensin II

F. J. Kaskel, P. Devarajan, L. A. Arbeit, J. S. Partin, L. C. Moore

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Cyclosporine-induced nephrotoxicity (CIN) was studied in rats treated for 7 days with cyclosporine (10 mg·kg-1·day-1 im) or vehicle (CON). CIN rats displayed characteristic reductions in glomerular filtration (GFR) and renal blood flow (RBF), and electron microscopy showed injury to proximal cells. Metabolic studies (7 day) showed significantly lower renal sodium excretion in conscious CIN rats compared with CON. In anesthetized rats at similar blood pressures, nephron GFR (SNGFR) was lower in CIN than CON, but fractional Na reabsorption was similar. In CIN, SNGFR, measured proximally to block flow to the sensing site of tubuloglomerular feedback (TGF) at the macula densa, was not significantly different than distal SNGFR. The rate of distal fluid delivery was significantly lower in CIN than in CON. Inhibition of the renin-angiotensin system (RAS) with captopril (CAP, 10 mg/kg iv), or saralasin (SAR, 0.3 mg·kg-1·h-1 iv) caused marked arterial hypotension in CIN and a fall in renal vascular resistance (RVR). With arterial pressure controlled, CAP or SAR increased GFR and RBF, and reduced RVR in CIN, but did not reverse the renal deficits compared with similarity treated CON. RBF autoregulation in CIN was impaired between 90 and 140 mmHg but was partially restored by CAP. We conclude 1) that both the filtered load and excretion rate of sodium in CIN are significantly reduced compared with controls, 2) that SNGFR in CIN is not depressed by TGF in response to elevated distal fluid delivery, and 3) that the RAS is not a primary mediator of the renal vasoconstriction in CIN.

Original languageEnglish (US)
Pages (from-to)F733-F742
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume252
Issue number4 (21/4)
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Physiology

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