Cyclin and caveolin expression in an acute model of murine chagasic myocarditis

Fnu Nagajyothi, Mahalia Desruisseaux, Boumediene Bouzahzah, Louis M. Weiss, Daniele Dos Santos Andrade, Stephen M. Factor, Philipp E. Scherer, Chris Albanese, Michael P. Lisanti, Herbert B. Tanowitz

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Chagas' disease caused by the parasite, Trypanosoma cruzi, is accompanied by an acute myocarditis which can be fatal. Mice (A/J strain) infected with T. cruzi (Tulahuen strain) develop an acute myocarditis associated with high parasitemia and uniform mortality. Examination of the myocardium demonstrated myonecrosis, vasculitis and parasite pseudocysts. Immunoblot analysis and quantitative real time PCR of heart lysates demonstrated an increased expression of cell cycle regulatory proteins such as cyclins B1, D1, A1 and E1 and an increased expression of cdk2 when compared with uninfecfed controls. Extracellular signal-regulated kinase (ERK) was activated. Proliferating cell nuclear antigen (PCNA), endothelin-1, endothelin receptor type A (ET A) and endothelin receptor type B (ETB) expression were increased. Caveolin-1 is important in the regulation of ERK and cyclin D1. The expression of caveolin-1 as well as caveolin-2 and caveolin-3 was reduced. These data suggest that acute fatal T. cruzi myocarditis is accompanied by changes in cell cycle proteins such as the cyclins and caveolin and that the upregulation of the endothelin pathway may be important in the myocardial abnormalities and mortality observed in this mouse model.

Original languageEnglish (US)
Pages (from-to)107-112
Number of pages6
JournalCell Cycle
Volume5
Issue number1
DOIs
StatePublished - Jan 1 2006

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Keywords

  • Caveolin
  • Cyclins
  • Endothelin-1
  • Myocarditis
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Nagajyothi, F., Desruisseaux, M., Bouzahzah, B., Weiss, L. M., Andrade, D. D. S., Factor, S. M., Scherer, P. E., Albanese, C., Lisanti, M. P., & Tanowitz, H. B. (2006). Cyclin and caveolin expression in an acute model of murine chagasic myocarditis. Cell Cycle, 5(1), 107-112. https://doi.org/10.4161/cc.5.1.2284