Cyclic nucleotide kinases and tachyzoite-bradyzoite transition in Toxoplasma gondii

Michael S. Eaton, Louis M. Weiss, Kami Kim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The ability of Toxoplasma gondii to cycle between the tachyzoite and bradyzoite life stages in intermediate hosts is key to parasite survival and the pathogenesis of toxoplasmosis. Studies from a number of laboratories indicate that differentiation in T. gondii is a stress-induced phenomenon. The signalling pathways or molecular mechanisms that control formation of the latent bradyzoite stage are unknown and specific effectors of differentiation have not been identified. We engineered a reporter parasite to facilitate simultaneous comparison of differentiation and replication after various treatments. Chloramphenicol acetyltransferase (CAT), expressed constitutively from the α-tubulin promoter (TUB1), was used to quantitate parasite number. β-galactosidase (β-GAL), expressed from a bradyzoite specific promoter (BAG1), was used as a measure of bradyzoite gene expression. Sodium nitroprusside, a well-known inducer of bradyzoite differentiation, reduced reporter parasite replication and caused bradyzoite differentiation. Stress-induced differentiation in many other pathogens is regulated by cyclic nucleotide kinases. Specific inhibitors of the cAMP dependent protein kinase and apicomplexan cGMP dependent protein kinase inhibited replication and induced differentiation. The β-GAL/CAT reporter parasite provides a method to quantify and compare agents that cause differentiation in T. gondii.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalInternational Journal for Parasitology
Volume36
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Cyclic Nucleotides
Toxoplasma
Parasites
Phosphotransferases
Chloramphenicol O-Acetyltransferase
Tubulin Modulators
Galactosidases
Cyclic GMP-Dependent Protein Kinases
Toxoplasmosis
Nitroprusside
Cyclic AMP-Dependent Protein Kinases
Gene Expression

Keywords

  • Bradyzoite
  • cAMP
  • cGMP
  • Cyclic nucleotide
  • Differentiation
  • Kinase
  • Reporter parasite
  • Toxoplasma

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Cyclic nucleotide kinases and tachyzoite-bradyzoite transition in Toxoplasma gondii. / Eaton, Michael S.; Weiss, Louis M.; Kim, Kami.

In: International Journal for Parasitology, Vol. 36, No. 1, 01.2006, p. 107-114.

Research output: Contribution to journalArticle

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