Cutting edge: Phosphatidylinositol 4,5-bisphosphate concentration at the APC side of the immunological synapse is required for effector T cell function

David Robert Fooksman, Saame Raza Shaikh, Sarah Boyle, Michael Edidin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Little is known about the signaling that occurs in an APC during contact with a T cell. In this article we report the concentration of the signaling lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at the APC side of the immunological synapse. In both human and mouse cells, a PI(4,5)P2-specific fluorescent reporter, PH-GFP (where PH is pleckstrin homology), detected an Ag-dependent enrichment of PI(4,5)P 2 at the synapse between Ag-specific T cells and APC. When PIP(4,5)P2 was sequestered by a high concentration of PH-GFP reporter, cells were less susceptible to CTL-mediated lysis than control cells. These findings suggest a new regulatory target for modulating immune function that may be exploited for immune escape by pathogens and tumors.

Original languageEnglish (US)
Pages (from-to)5179-5182
Number of pages4
JournalJournal of Immunology
Volume182
Issue number9
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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