Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats

Xing Guangwei, Lu Rongzhu, Xu Wenrong, Wang Suhua, Zhao Xiaowu, Wang Shizhong, Zhang Ye, Michael Aschner, Shrinivas K. Kulkarni, Mahendra Bishnoi

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Acrylonitrile (AN) is widely used in the manufacturing of fibers, plastics and pharmaceuticals. Free radical-mediated lipid peroxidation is implicated in the toxicity of AN. The present study was designed to examine the ability of curcumin, a natural polyphenolic compound, to attenuate acute AN-induced lipid peroxidation in the brain and liver of rats. Male Sprague-Dawley rats were orally administered curcumin at doses of 0 (olive oil control), 50 or 100 mg/kg bodyweight daily for 7 consecutive days. Two hours after the last dose of curcumin, rats received an intraperitoneal injection of 50 mg AN/kg bodyweight. Acute exposure to AN significantly increased the generation of lipid peroxidation products, reflected by high levels of malondialdehyde (MDA) both in the brain and liver. These increases were accompanied by a significant decrease in reduced glutathione (GSH) content and a significant reduction in catalase (CAT) activity in the same tissues. No consistent changes in superoxide dismutase (SOD) activity were observed between the control and AN-treatment groups in both tissues. Pretreatment with curcumin reversed the AN-induced effects, reducing the levels of MDA and enhancing CAT activity and increasing reduced GSH content both in the brain and liver. Furthermore, curcumin effectively prevented AN-induced decrease in cytochrome c oxidase activity in both liver and brain. These results establish that curcumin pretreatment has a beneficial role in mitigating AN-induced oxidative stress both in the brains and livers of exposed rats and these effects are mediated independently of cytochrome P450 2E1 inhibition. Accordingly, curcumin should be considered as a potential safe and effective approach in attenuating the adverse effects produced by AN-related toxicants.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalToxicology
Volume267
Issue number1-3
DOIs
StatePublished - Jan 12 2010
Externally publishedYes

Fingerprint

Acrylonitrile
Curcumin
Rats
Liver
Brain
Lipid Peroxidation
Malondialdehyde
Lipids
Catalase
Polyphenolic compounds
Tissue
Cytochrome P-450 CYP2E1
Oxidative stress
Electron Transport Complex IV
Intraperitoneal Injections
Plastics
Superoxide Dismutase
Free Radicals
Glutathione
Sprague Dawley Rats

Keywords

  • Acrylonitrile
  • Curcumin
  • Cytochrome c oxidase
  • Lipid peroxidation

ASJC Scopus subject areas

  • Toxicology

Cite this

Guangwei, X., Rongzhu, L., Wenrong, X., Suhua, W., Xiaowu, Z., Shizhong, W., ... Bishnoi, M. (2010). Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. Toxicology, 267(1-3), 140-146. https://doi.org/10.1016/j.tox.2009.11.001

Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. / Guangwei, Xing; Rongzhu, Lu; Wenrong, Xu; Suhua, Wang; Xiaowu, Zhao; Shizhong, Wang; Ye, Zhang; Aschner, Michael; Kulkarni, Shrinivas K.; Bishnoi, Mahendra.

In: Toxicology, Vol. 267, No. 1-3, 12.01.2010, p. 140-146.

Research output: Contribution to journalArticle

Guangwei, X, Rongzhu, L, Wenrong, X, Suhua, W, Xiaowu, Z, Shizhong, W, Ye, Z, Aschner, M, Kulkarni, SK & Bishnoi, M 2010, 'Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats', Toxicology, vol. 267, no. 1-3, pp. 140-146. https://doi.org/10.1016/j.tox.2009.11.001
Guangwei X, Rongzhu L, Wenrong X, Suhua W, Xiaowu Z, Shizhong W et al. Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. Toxicology. 2010 Jan 12;267(1-3):140-146. https://doi.org/10.1016/j.tox.2009.11.001
Guangwei, Xing ; Rongzhu, Lu ; Wenrong, Xu ; Suhua, Wang ; Xiaowu, Zhao ; Shizhong, Wang ; Ye, Zhang ; Aschner, Michael ; Kulkarni, Shrinivas K. ; Bishnoi, Mahendra. / Curcumin pretreatment protects against acute acrylonitrile-induced oxidative damage in rats. In: Toxicology. 2010 ; Vol. 267, No. 1-3. pp. 140-146.
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