Cultured astrocytes and neurons synthesize and secrete carboxypeptidase E, and neuropeptide-processing enzyme

M. H. Vilijn, B. Das, J. A. Kessler, Lloyd D. Fricker

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Carboxypeptidase E (EC 3.4.17.10) is a carboxypeptidase B-like enzyme associated with the biosynthesis of many peptide hormones and neurotransmitters. Media collected from cultured astrocytes contain a carboxypeptidase E-like activity. Cultured astrocytes secrete ~73% of their cellular level of carboxypeptidase E per hour, and secretion is not substantially influenced by 35 mM KCl. In contrast, neurons secrete only 29% of their cellular carboxypeptidase E per hour, but secretion increases to 86% on stimulation with 35 mM KCl. Secretion of carboxypeptidase E activity from both neuronal and astrocyte cultures is relatively selective; neither acid phosphatase or acetylglucosaminidase is secreted in appreciable amounts. Cultured neurons and astrocytes express a carboxypeptidase E mRNA of a similar size. The levels of this mRNA differ in astrocytes cultured from different brain regions, with high levels in striatal, cortical, hippocampal, and hypothalamic astrocytes and low levels in cerebellar astrocytes. The level of carboxypeptidase E mRNA in hypothalamic astrocyte cultures is four- to fivefold higher than the level in hypothalamic neuronal cultures. These results indicate that cultured astrocytes express carboxypeptidase E mRNA and enzymatic activity and thus contain one of the enzymes required in the biosynthesis of many peptide hormones and neurotransmitters.

Original languageEnglish (US)
Pages (from-to)1487-1493
Number of pages7
JournalJournal of Neurochemistry
Volume53
Issue number5
StatePublished - 1989

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Carboxypeptidase H
Neuropeptides
Astrocytes
Neurons
Enzymes
Processing
Messenger RNA
Peptide Hormones
Biosynthesis
Neurotransmitter Agents
Carboxypeptidase B
Corpus Striatum
Acetylglucosaminidase
Acid Phosphatase
Brain

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Cultured astrocytes and neurons synthesize and secrete carboxypeptidase E, and neuropeptide-processing enzyme. / Vilijn, M. H.; Das, B.; Kessler, J. A.; Fricker, Lloyd D.

In: Journal of Neurochemistry, Vol. 53, No. 5, 1989, p. 1487-1493.

Research output: Contribution to journalArticle

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