Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia, Ann Kindt, Hernan Bermudez, Alejandro Llanos-Cuentas, Simonne De Doncker, Jorge Arevalo, Kelly Wilber Quispe Tintaya, Jean Claude Dujardin

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Original languageEnglish (US)
Pages (from-to)2294-2297
Number of pages4
JournalJournal of Clinical Microbiology
Volume42
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

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Epidemiological Monitoring
HSP70 Heat-Shock Proteins
Leishmaniasis
Trypanosoma cruzi
Leishmania
Capillary Electrophoresis
Restriction Fragment Length Polymorphisms
Equipment and Supplies
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes. / Garcia, Lineth; Kindt, Ann; Bermudez, Hernan; Llanos-Cuentas, Alejandro; De Doncker, Simonne; Arevalo, Jorge; Tintaya, Kelly Wilber Quispe; Dujardin, Jean Claude.

In: Journal of Clinical Microbiology, Vol. 42, No. 5, 05.2004, p. 2294-2297.

Research output: Contribution to journalArticle

Garcia, L, Kindt, A, Bermudez, H, Llanos-Cuentas, A, De Doncker, S, Arevalo, J, Tintaya, KWQ & Dujardin, JC 2004, 'Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes', Journal of Clinical Microbiology, vol. 42, no. 5, pp. 2294-2297. https://doi.org/10.1128/JCM.42.5.2294-2297.2004
Garcia, Lineth ; Kindt, Ann ; Bermudez, Hernan ; Llanos-Cuentas, Alejandro ; De Doncker, Simonne ; Arevalo, Jorge ; Tintaya, Kelly Wilber Quispe ; Dujardin, Jean Claude. / Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes. In: Journal of Clinical Microbiology. 2004 ; Vol. 42, No. 5. pp. 2294-2297.
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