Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia, Ann Kindt, Hernan Bermudez, Alejandro Llanos-Cuentas, Simonne De Doncker, Jorge Arevalo, Kelly Wilber Quispe Tintaya, Jean Claude Dujardin

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Original languageEnglish (US)
Pages (from-to)2294-2297
Number of pages4
JournalJournal of Clinical Microbiology
Volume42
Issue number5
DOIs
StatePublished - May 1 2004

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes'. Together they form a unique fingerprint.

  • Cite this

    Garcia, L., Kindt, A., Bermudez, H., Llanos-Cuentas, A., De Doncker, S., Arevalo, J., Tintaya, K. W. Q., & Dujardin, J. C. (2004). Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes. Journal of Clinical Microbiology, 42(5), 2294-2297. https://doi.org/10.1128/JCM.42.5.2294-2297.2004