Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia, Ann Kindt, Hernan Bermudez, Alejandro Llanos-Cuentas, Simonne De Doncker, Jorge Arevalo, Kelly Wilber Quispe Tintaya, Jean Claude Dujardin

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Abstract

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Original languageEnglish (US)
Pages (from-to)2294-2297
Number of pages4
JournalJournal of Clinical Microbiology
Volume42
Issue number5
DOIs
Publication statusPublished - May 1 2004

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ASJC Scopus subject areas

  • Microbiology (medical)

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