TY - JOUR
T1 - Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes
AU - Garcia, Lineth
AU - Kindt, Ann
AU - Bermudez, Hernan
AU - Llanos-Cuentas, Alejandro
AU - De Doncker, Simonne
AU - Arevalo, Jorge
AU - Tintaya, Kelly Wilber Quispe
AU - Dujardin, Jean Claude
PY - 2004/5
Y1 - 2004/5
N2 - PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.
AB - PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.
UR - http://www.scopus.com/inward/record.url?scp=2442551896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442551896&partnerID=8YFLogxK
U2 - 10.1128/JCM.42.5.2294-2297.2004
DO - 10.1128/JCM.42.5.2294-2297.2004
M3 - Article
C2 - 15131217
AN - SCOPUS:2442551896
SN - 0095-1137
VL - 42
SP - 2294
EP - 2297
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -