CSF-1 stimulated association ofcbl with signaling proteins in macrophages

Yarong Wang, Y. G. Yeung, E. R. Stanley

Research output: Contribution to journalArticle

Abstract

We have reported that CSF-1 stimulation of macrophages leads to the transient tyrosine phosphorylalion, membrane localization and ubiquitination of the proto-oncogene product Cbl. To further study the kinetic relationships among these events, we stimulated cells for various times with CSF-1 at 4°C and analyzed Cbl immunoprecipiiations from cytosolic and membrane fractions. As early as 10 min of stimulation, Cbl was tyrosine phosphorylated and translocated from cytosolic to membrane fraction where it associated with CSF-1 R, Sos, p85 subunit of PI3 Kinase, She and an increased amount of Grb2. At about l h of stimulation Cbl ubiquitination was detected in the membrane fraction, and some of the phosphorylated and ubquitinated Cbl was also found in the cytosolic fraction. The association of Cbl with She and Grb2 persisted over 5 h of stimulation while the association with CSF-1R, Sos and some of p85 was transient and decreased over 20-40 min. Our results suggest that tyrosine phosphorylation, preceding ubquitination, is associated with Cbl translocation and association with signaling proteins in the membrane fraction. The function of Cbl ubquitination is not clear. However this kinetic analysis indicates that ubquitination of Cbl could be important for its dissociation from those signaling proteins and/or its movement back from the membrane to the cytoplasm.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

Fingerprint

Macrophage Colony-Stimulating Factor
Macrophages
tyrosine
macrophages
Association reactions
Membranes
Tyrosine
Ubiquitination
proto-oncogenes
kinetics
Proteins
proteins
membrane proteins
phosphorylation
phosphotransferases (kinases)
cytoplasm
Proto-Oncogenes
Oncogene Proteins
Phosphorylation
Kinetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Wang, Y., Yeung, Y. G., & Stanley, E. R. (1997). CSF-1 stimulated association ofcbl with signaling proteins in macrophages. FASEB Journal, 11(9).

CSF-1 stimulated association ofcbl with signaling proteins in macrophages. / Wang, Yarong; Yeung, Y. G.; Stanley, E. R.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Wang, Y, Yeung, YG & Stanley, ER 1997, 'CSF-1 stimulated association ofcbl with signaling proteins in macrophages', FASEB Journal, vol. 11, no. 9.
Wang, Yarong ; Yeung, Y. G. ; Stanley, E. R. / CSF-1 stimulated association ofcbl with signaling proteins in macrophages. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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