Crystals of a thymidylate synthase mutant offer insights into crystal packing and plasticity of protein-protein contacts

B. Gopal, V. Prasanna, S. Parthasarathy, D. V. Santi, P. Balaram, M. R N Murthy

Research output: Contribution to journalArticle

Abstract

Some crystal forms of thymidylate synthasc from L. casei exhibit unit cell transformation upon irradiation by X-rays. These forms, all of which occur in the space group P6122, show an elongation in the c cell dimension and in some cases stabilize to a constant cell dimension upon prolonged exposure. We present here an analysis of the possible causes of this transformation based on the crystal structures for two forms of an R178F mutant of this enzyme. We compare these structures to other structures with intermediate cell parameters reported in the literature. There are no large changes in the dimeric structure of TS in these crystal forms. Although there is a large change in the unit cell volume, the molecular contacts in the crystal structures are nearly invariant. The transformation appears to result from concerted small changes in molecular structure and intermolecular contacts. These observations corroborate the general impression that protein structures can accommodate minor changes in sequence or packing wherein the intra and intermolecular interactions are not seriously altered.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalCurrent Science
Volume75
Issue number3
StatePublished - Aug 10 1998
Externally publishedYes

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thymidylate synthase
Thymidylate Synthase
crystals
mutants
crystal structure
Proteins
proteins
cells
Molecular Structure
Cell Size
protein structure
chemical structure
X-Rays
X-radiation
irradiation
Enzymes
enzymes

ASJC Scopus subject areas

  • General
  • Agricultural and Biological Sciences(all)

Cite this

Gopal, B., Prasanna, V., Parthasarathy, S., Santi, D. V., Balaram, P., & Murthy, M. R. N. (1998). Crystals of a thymidylate synthase mutant offer insights into crystal packing and plasticity of protein-protein contacts. Current Science, 75(3), 299-304.

Crystals of a thymidylate synthase mutant offer insights into crystal packing and plasticity of protein-protein contacts. / Gopal, B.; Prasanna, V.; Parthasarathy, S.; Santi, D. V.; Balaram, P.; Murthy, M. R N.

In: Current Science, Vol. 75, No. 3, 10.08.1998, p. 299-304.

Research output: Contribution to journalArticle

Gopal, B, Prasanna, V, Parthasarathy, S, Santi, DV, Balaram, P & Murthy, MRN 1998, 'Crystals of a thymidylate synthase mutant offer insights into crystal packing and plasticity of protein-protein contacts', Current Science, vol. 75, no. 3, pp. 299-304.
Gopal B, Prasanna V, Parthasarathy S, Santi DV, Balaram P, Murthy MRN. Crystals of a thymidylate synthase mutant offer insights into crystal packing and plasticity of protein-protein contacts. Current Science. 1998 Aug 10;75(3):299-304.
Gopal, B. ; Prasanna, V. ; Parthasarathy, S. ; Santi, D. V. ; Balaram, P. ; Murthy, M. R N. / Crystals of a thymidylate synthase mutant offer insights into crystal packing and plasticity of protein-protein contacts. In: Current Science. 1998 ; Vol. 75, No. 3. pp. 299-304.
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