Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis

Aneesh Goyal, Malikmohamed Yousuf, Eerappa Rajakumara, Pooja Arora, Rajesh S. Gokhale, Rajan Sankaranarayanan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

FadD28 from Mycobacterium tuberculosis belongs to the fatty-acyl AMP ligase (FAAL) family of proteins. It is essential for the biosynthesis of a virulent phthiocerol dimycocerosate (PDIM) lipid that is only found in the cell wall of pathogenic mycobacteria. The N-terminal domain, comprising of the first 460 residues, was crystallized by the hanging-drop vapour-diffusion method at 295 K. The crystals belong to space group P212121, with unit-cell parameters a = 50.97, b = 60.74, c = 136.54 Å. The crystal structure of the N-terminal domain of FadD28 at 2.35 Å resolution has been solved using the MAD method.

Original languageEnglish (US)
Pages (from-to)350-352
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume62
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

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adenosine monophosphate
tuberculosis
Biosynthesis
Adenosine Monophosphate
Ligases
Crystallization
Mycobacterium tuberculosis
Crystal structure
Vapors
Cells
X-Rays
crystallization
Lipids
X rays
Crystals
biosynthesis
Mycobacterium
Cell Wall
lipids
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis. / Goyal, Aneesh; Yousuf, Malikmohamed; Rajakumara, Eerappa; Arora, Pooja; Gokhale, Rajesh S.; Sankaranarayanan, Rajan.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 4, 04.2006, p. 350-352.

Research output: Contribution to journalArticle

Goyal, Aneesh ; Yousuf, Malikmohamed ; Rajakumara, Eerappa ; Arora, Pooja ; Gokhale, Rajesh S. ; Sankaranarayanan, Rajan. / Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2006 ; Vol. 62, No. 4. pp. 350-352.
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