Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis

Aneesh Goyal; Malikmohamed Yousuf; Eerappa Rajakumara; Pooja Arora; Rajesh S. Gokhale; Rajan Sankaranarayanan

doi:10.1107/S1744309106005938

Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis

Aneesh Goyal, Malikmohamed Yousuf, Eerappa Rajakumara, Pooja Arora, Rajesh S. Gokhale, Rajan Sankaranarayanan

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

FadD28 from Mycobacterium tuberculosis belongs to the fatty-acyl AMP ligase (FAAL) family of proteins. It is essential for the biosynthesis of a virulent phthiocerol dimycocerosate (PDIM) lipid that is only found in the cell wall of pathogenic mycobacteria. The N-terminal domain, comprising of the first 460 residues, was crystallized by the hanging-drop vapour-diffusion method at 295 K. The crystals belong to space group P2₁2₁2₁, with unit-cell parameters a = 50.97, b = 60.74, c = 136.54 Å. The crystal structure of the N-terminal domain of FadD28 at 2.35 Å resolution has been solved using the MAD method.

Original language	English (US)
Pages (from-to)	350-352
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	62
Issue number	4
DOIs	https://doi.org/10.1107/S1744309106005938
State	Published - Apr 2006
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1107/S1744309106005938

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Goyal, A., Yousuf, M., Rajakumara, E., Arora, P., Gokhale, R. S., & Sankaranarayanan, R. (2006). Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 62(4), 350-352. https://doi.org/10.1107/S1744309106005938

Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis. / Goyal, Aneesh; Yousuf, Malikmohamed; Rajakumara, Eerappa et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 4, 04.2006, p. 350-352.

Research output: Contribution to journal › Article › peer-review

Goyal, A, Yousuf, M, Rajakumara, E, Arora, P, Gokhale, RS & Sankaranarayanan, R 2006, 'Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 62, no. 4, pp. 350-352. https://doi.org/10.1107/S1744309106005938

Goyal A, Yousuf M, Rajakumara E, Arora P, Gokhale RS, Sankaranarayanan R. Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2006 Apr;62(4):350-352. doi: 10.1107/S1744309106005938

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abstract = "FadD28 from Mycobacterium tuberculosis belongs to the fatty-acyl AMP ligase (FAAL) family of proteins. It is essential for the biosynthesis of a virulent phthiocerol dimycocerosate (PDIM) lipid that is only found in the cell wall of pathogenic mycobacteria. The N-terminal domain, comprising of the first 460 residues, was crystallized by the hanging-drop vapour-diffusion method at 295 K. The crystals belong to space group P212121, with unit-cell parameters a = 50.97, b = 60.74, c = 136.54 {\AA}. The crystal structure of the N-terminal domain of FadD28 at 2.35 {\AA} resolution has been solved using the MAD method.",

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Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis

Abstract

ASJC Scopus subject areas

UN SDGs

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