Crystallization and preliminary X-ray analysis of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus

Matthew W. Vetting, Cathleen A. Earhart, Douglas H. Ohlendorf

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 Å. The dodecahedral crystals diffract to beyond 2.5 Å resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe+3)12 complex.

Original languageEnglish (US)
Pages (from-to)372-373
Number of pages2
JournalJournal of Molecular Biology
Volume236
Issue number1
DOIs
StatePublished - Feb 10 1994

Keywords

  • Acinetobacter calcoaceticus
  • Crystallization
  • Metalloenzymes
  • Protocatechuate 3,4-dioxygenase
  • X-ray diffraction

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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