Crystallization and preliminary x-ray analysis of drosophila glutathione S-transferase-2

Bogos Agianian, J. D. Clayton, K. Leonard, P. Tucker, B. Bullard, P. Gros

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The σ-class glutathione S-transferase-2 (GST-2) from Drosophila melanogaster is predominantly found within the indirect flight muscles (IFMs), where it is bound to the 'heavy' subunit of the IFM thin filament troponin complex (Tn-H). An N-terminal extension found in GST-2 is unique within the σ GST class and may be involved in its interaction with Tn-H or modulate its enzymatic function. The recombinant protein has been crystallized at room temperature using ammonium sulfate as precipitant. Synchrotron radiation was used to measure a complete native data set to 1.75 Å resolution from flash-cooled crystals. The crystals belong to one of the trigonal space groups P3121 or P3221, with unit-cell parameters a = b = 89.7, c = 131.8 Å. The self-rotation function is consistent with a GST-2 dimer in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)725-727
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Drosophila
x ray analysis
glutathione
Crystallization
Glutathione Transferase
X-Rays
crystallization
muscles
X rays
Muscle
flight
Muscles
Crystals
Synchrotrons
ammonium sulfates
Troponin
Ammonium Sulfate
Synchrotron radiation
Drosophila melanogaster
Recombinant Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary x-ray analysis of drosophila glutathione S-transferase-2. / Agianian, Bogos; Clayton, J. D.; Leonard, K.; Tucker, P.; Bullard, B.; Gros, P.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 57, No. 5, 2001, p. 725-727.

Research output: Contribution to journalArticle

Agianian, Bogos ; Clayton, J. D. ; Leonard, K. ; Tucker, P. ; Bullard, B. ; Gros, P. / Crystallization and preliminary x-ray analysis of drosophila glutathione S-transferase-2. In: Acta Crystallographica Section D: Biological Crystallography. 2001 ; Vol. 57, No. 5. pp. 725-727.
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