Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nα-acetylation of ribosomal protein S18

Matthew W. Vetting, David C. Bareich, Michael Yu, John S. Blanchard

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The three ribosomal proteins L7, S5, and S18 are included in the rare subset of prokaryotic proteins that are known to be Nα- acetylated. The GCN5-related N-acetyltransferase (GNAT) protein RimI, responsible for the Nα-acetylation of the ribosomal protein S18, was cloned from Salmonella typhimurium LT2 (RimIST), overexpressed, and purified to homogeneity. Steady-state kinetic parameters for RimIST were determined for AcCoA and a peptide substrate consisting of the first six amino acids of the target protein S18. The crystal structure of RimIST was determined in complex with CoA, AcCoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor. The structures are consistent with a direct nucleophilic addition-elimination mechanism with Glu103 and Tyr115 acting as the catalytic base and acid, respectively. The RimIST-bisubstrate complex suggests that several residues change conformation upon interacting with the N terminus of S18, including Glu103, the proposed active site base, facilitating proton exchange and catalysis.

Original languageEnglish (US)
Pages (from-to)1781-1790
Number of pages10
JournalProtein Science
Volume17
Issue number10
DOIs
StatePublished - Oct 2008

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Acetylation
Acetyltransferases
Salmonella
Salmonella typhimurium
Crystal structure
Coenzyme A
Acetyl Coenzyme A
Proteins
Ribosomal Proteins
Catalysis
Kinetic parameters
Conformations
Protons
Catalytic Domain
Ion exchange
Amino Acids
Peptides
Acids
Substrates
ribosomal protein S18

Keywords

  • Bisubstrate inhibition
  • GNAT structure
  • Protein N-acetylation
  • Ribosomal protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nα-acetylation of ribosomal protein S18. / Vetting, Matthew W.; Bareich, David C.; Yu, Michael; Blanchard, John S.

In: Protein Science, Vol. 17, No. 10, 10.2008, p. 1781-1790.

Research output: Contribution to journalArticle

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