Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

Michael Soniat, Parthasarathy Sampathkumar, Garen Collett, Anthony S. Gizzi, Radhika N. Banu, Rahul C. Bhosle, Swetha Chamala, Sukanya Chowdhury, Andras Fiser, Alan S. Glenn, James Hammonds, Brandan Hillerich, Kamil Khafizov, James D. Love, Bridget Matikainen, Ronald D. Seidel, Rafael Toro, P. Rajesh Kumar, Jeffrey B. Bonanno, Yuh Min Chook & 1 others Steven C. Almo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalJournal of Structural and Functional Genomics
Volume14
Issue number2
DOIs
StatePublished - Jun 2013

Fingerprint

Karyopherins
Nuclear Localization Signals
Yeast
Saccharomyces cerevisiae
Crystal structure
RNA-Binding Proteins
Nuclear Proteins
Conformations
Proteins
Binding Sites
Cells
Nuclear Pore
Messenger RNA
Processing
Eukaryota
Cell Nucleus

Keywords

  • Importin
  • Karyopherin
  • Nab2
  • Nuclear import
  • Nuclear localization signal
  • Nuclear pore
  • Nucleocytoplasmic transport

ASJC Scopus subject areas

  • Genetics
  • Structural Biology
  • Biochemistry

Cite this

Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2. / Soniat, Michael; Sampathkumar, Parthasarathy; Collett, Garen; Gizzi, Anthony S.; Banu, Radhika N.; Bhosle, Rahul C.; Chamala, Swetha; Chowdhury, Sukanya; Fiser, Andras; Glenn, Alan S.; Hammonds, James; Hillerich, Brandan; Khafizov, Kamil; Love, James D.; Matikainen, Bridget; Seidel, Ronald D.; Toro, Rafael; Rajesh Kumar, P.; Bonanno, Jeffrey B.; Chook, Yuh Min; Almo, Steven C.

In: Journal of Structural and Functional Genomics, Vol. 14, No. 2, 06.2013, p. 31-35.

Research output: Contribution to journalArticle

Soniat, M, Sampathkumar, P, Collett, G, Gizzi, AS, Banu, RN, Bhosle, RC, Chamala, S, Chowdhury, S, Fiser, A, Glenn, AS, Hammonds, J, Hillerich, B, Khafizov, K, Love, JD, Matikainen, B, Seidel, RD, Toro, R, Rajesh Kumar, P, Bonanno, JB, Chook, YM & Almo, SC 2013, 'Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2', Journal of Structural and Functional Genomics, vol. 14, no. 2, pp. 31-35. https://doi.org/10.1007/s10969-013-9150-1
Soniat, Michael ; Sampathkumar, Parthasarathy ; Collett, Garen ; Gizzi, Anthony S. ; Banu, Radhika N. ; Bhosle, Rahul C. ; Chamala, Swetha ; Chowdhury, Sukanya ; Fiser, Andras ; Glenn, Alan S. ; Hammonds, James ; Hillerich, Brandan ; Khafizov, Kamil ; Love, James D. ; Matikainen, Bridget ; Seidel, Ronald D. ; Toro, Rafael ; Rajesh Kumar, P. ; Bonanno, Jeffrey B. ; Chook, Yuh Min ; Almo, Steven C. / Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2. In: Journal of Structural and Functional Genomics. 2013 ; Vol. 14, No. 2. pp. 31-35.
@article{d76e882f661f4204b9685bc2377cfd55,
title = "Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2",
abstract = "Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-{\AA} resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.",
keywords = "Importin, Karyopherin, Nab2, Nuclear import, Nuclear localization signal, Nuclear pore, Nucleocytoplasmic transport",
author = "Michael Soniat and Parthasarathy Sampathkumar and Garen Collett and Gizzi, {Anthony S.} and Banu, {Radhika N.} and Bhosle, {Rahul C.} and Swetha Chamala and Sukanya Chowdhury and Andras Fiser and Glenn, {Alan S.} and James Hammonds and Brandan Hillerich and Kamil Khafizov and Love, {James D.} and Bridget Matikainen and Seidel, {Ronald D.} and Rafael Toro and {Rajesh Kumar}, P. and Bonanno, {Jeffrey B.} and Chook, {Yuh Min} and Almo, {Steven C.}",
year = "2013",
month = "6",
doi = "10.1007/s10969-013-9150-1",
language = "English (US)",
volume = "14",
pages = "31--35",
journal = "Journal of Structural and Functional Genomics",
issn = "1345-711X",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

AU - Soniat, Michael

AU - Sampathkumar, Parthasarathy

AU - Collett, Garen

AU - Gizzi, Anthony S.

AU - Banu, Radhika N.

AU - Bhosle, Rahul C.

AU - Chamala, Swetha

AU - Chowdhury, Sukanya

AU - Fiser, Andras

AU - Glenn, Alan S.

AU - Hammonds, James

AU - Hillerich, Brandan

AU - Khafizov, Kamil

AU - Love, James D.

AU - Matikainen, Bridget

AU - Seidel, Ronald D.

AU - Toro, Rafael

AU - Rajesh Kumar, P.

AU - Bonanno, Jeffrey B.

AU - Chook, Yuh Min

AU - Almo, Steven C.

PY - 2013/6

Y1 - 2013/6

N2 - Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

AB - Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

KW - Importin

KW - Karyopherin

KW - Nab2

KW - Nuclear import

KW - Nuclear localization signal

KW - Nuclear pore

KW - Nucleocytoplasmic transport

UR - http://www.scopus.com/inward/record.url?scp=84879234011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879234011&partnerID=8YFLogxK

U2 - 10.1007/s10969-013-9150-1

DO - 10.1007/s10969-013-9150-1

M3 - Article

VL - 14

SP - 31

EP - 35

JO - Journal of Structural and Functional Genomics

JF - Journal of Structural and Functional Genomics

SN - 1345-711X

IS - 2

ER -