Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

Michael Soniat, Parthasarathy Sampathkumar, Garen Collett, Anthony S. Gizzi, Radhika N. Banu, Rahul C. Bhosle, Swetha Chamala, Sukanya Chowdhury, Andras Fiser, Alan S. Glenn, James Hammonds, Brandan Hillerich, Kamil Khafizov, James D. Love, Bridget Matikainen, Ronald D. Seidel, Rafael Toro, P. Rajesh Kumar, Jeffery B. Bonanno, Yuh Min ChookSteven C. Almo

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalJournal of Structural and Functional Genomics
Volume14
Issue number2
DOIs
StatePublished - Jun 1 2013

Keywords

  • Importin
  • Karyopherin
  • Nab2
  • Nuclear import
  • Nuclear localization signal
  • Nuclear pore
  • Nucleocytoplasmic transport

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

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    Soniat, M., Sampathkumar, P., Collett, G., Gizzi, A. S., Banu, R. N., Bhosle, R. C., Chamala, S., Chowdhury, S., Fiser, A., Glenn, A. S., Hammonds, J., Hillerich, B., Khafizov, K., Love, J. D., Matikainen, B., Seidel, R. D., Toro, R., Rajesh Kumar, P., Bonanno, J. B., ... Almo, S. C. (2013). Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2. Journal of Structural and Functional Genomics, 14(2), 31-35. https://doi.org/10.1007/s10969-013-9150-1