Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii

Shenping Liu, Alexander A. Fedorov, Thomas D. Pollard, Eaton Edward Lattman, Steven C. Almo, Karen A. Magnus

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Profilin-I from Acanthamoeba castellanii is a 13-kDa protein that binds actin and poly-L-proline. The native protein has been crystallized in two different but closely related forms. The second form proved more amenable to three-dimensional structural determination using heavy-atom isomorphous methods to obtain crystallographic phase information. We used the second crystal structure as a test molecule in the molecular replacement procedure to determine the structure of the first crystal form of profilin-I. More residues participate in crystal lattice contacts in the first crystal form than in the second. The two crystal forms differ significantly in the C- terminal helix that interacts with actin and in the loop preceding this helix. Coordinates of some main chain atoms here differ by about 1.0 Å, and side chain atoms differ by more than 2.0 Å.

Original languageEnglish (US)
Pages (from-to)22-29
Number of pages8
JournalJournal of Structural Biology
Volume123
Issue number1
DOIs
StatePublished - Sep 1998

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Acanthamoeba castellanii
Profilins
Actins
Proteins

Keywords

  • Actin cytoskeleton
  • Crystal structure
  • Isomorphous replacement
  • Phosphoinositol-based signaling
  • Profilin

ASJC Scopus subject areas

  • Structural Biology

Cite this

Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii. / Liu, Shenping; Fedorov, Alexander A.; Pollard, Thomas D.; Lattman, Eaton Edward; Almo, Steven C.; Magnus, Karen A.

In: Journal of Structural Biology, Vol. 123, No. 1, 09.1998, p. 22-29.

Research output: Contribution to journalArticle

Liu, Shenping ; Fedorov, Alexander A. ; Pollard, Thomas D. ; Lattman, Eaton Edward ; Almo, Steven C. ; Magnus, Karen A. / Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii. In: Journal of Structural Biology. 1998 ; Vol. 123, No. 1. pp. 22-29.
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