Cryptococcus neoformans responds to mannitol by increasing capsule size in vitro and in vivo

Allan Jefferson Guimarães, Susana Frases, Radamés J.B. Cordero, Leonardo Nimrichter, Arturo Casadevall, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The polysaccharide capsule of the fungus Cryptococcus neoformans is its main virulence factor. In this study, we determined the effects of mannitol and glucose on the capsule and exopolysaccharide production. Growth in mannitol significantly increased capsular volume compared with cultivation in glucose. However, cells grown in glucose concentrations higher than 62.5 mM produced more exopolysaccharide than cells grown in mannitol. The fibre lengths and glycosyl composition of capsular polysaccharide from yeast grown in mannitol was structurally different from that of yeast grown in glucose. Furthermore, mannitol treatment of mice infected intratracheally with C. neoformans resulted in fungal cells with significantly larger capsules and the mice had reduced fungal dissemination to the brain. Our results demonstrate the capacity of carbohydrate source and concentration to modify the expression of amajor virulence factor of C. neoformans. These findings may impact the clinical management of cryptococcosis.

Original languageEnglish (US)
Pages (from-to)740-753
Number of pages14
JournalCellular Microbiology
Volume12
Issue number6
DOIs
StatePublished - Jun 2010

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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